Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Transferase other than ribonuclease
Patent
1995-06-07
1998-06-16
Prouty, Rebecca E.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Transferase other than ribonuclease
435 703, 4351723, 435325, 435358, 4352523, 4353201, 536 231, 536 232, 935 14, 935 22, 935 27, 935 66, C12N 1554, C12N 910, C12N 510, C12N 1563
Patent
active
057669104
ABSTRACT:
The present invention provides an isolated nucleic acid molecule encoding both a soluble and membrane-bound human .beta.-1,6-N-acetylglucosaminyltransferase, the I-branching enzyme (IGnT). The invention also provides vectors containing the isolated nucleic acid molecule encoding human IGnT as well as recombinant host cells transformed with the vectors. The invention further provides a method of preparing a membrane-bound form of human IGnT and methods of preparing and purifying soluble human IGnT and active fragments of either form. Also provided are antisense oligonucleotides complementary to a nucleic acid molecule encoding a human IGnT or an active fragment thereof, antibodies directed to the human IGnT, pharmaceutical compositions related to the human IGnT and transgenic nonhuman mammals expressing DNA encoding normal or mutant human IGnT. Also provided are methods for regulating the expression of human IGnT and methods for modifying a biological function mediated by the regulatory activity of human IGnT. Methods of detecting the presence of linear polylactosaminoglycans expressing i antigenic determinants on a cell surface also are provided.
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Bierhuizen, Marti F.A. et al. "Expression of the Developmental I Antigen by a Cloned Human cDNA Encoding a Member of a .beta.-1, 6-N-Acetylglucosaminyltransferase Gene Family." Genes and Development. 7:468-478 (1993).
Bierhuizen Marti F. A.
Fukuda Minoru
La Jolla Cancer Research Foundation
Prouty Rebecca E.
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