Expression of sucrose phosphorylase in plants

Chemistry: molecular biology and microbiology – Spore forming or isolating process

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800205, 800DIG42, 800DIG43, 800DIG52, 800DIG27, 800DIG32, 800DIG66, 536 232, 536 237, 435 691, 4351904, 4351723, 435101, C12N 1529, C12N 1531, A01H 400, A01H 500

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057168377

ABSTRACT:
Introducing sucrose phosphorylase activity into plants by transformation with a gene for the enzyme increases the rate of sucrose hydrolysis, leading to increased starch, oil, and protein levels. The preferred gene is from Streptococcus mutans. Surprisingly, in potatoes transformed to express this gene in tubers, reduced bruise discoloration susceptibility and increased uniformity of starch deposition throughout the tuber are achieved.

REFERENCES:
patent: 4888170 (1989-12-01), Curtiss, III
patent: 5349123 (1994-09-01), Shewmaker et al.
Russell et al. Streptococcus mutans gftA Gene Specifies Sucrose Phosphorylase. Infection and Immunity. pp. 2763-2765, Oct. 1988.
Ferratti et al. Sequence Analysis of the Glucosyltransferase A Gene (gftA) from Streptococcus mutans Ingbritt. Infection and Immunity pp. 1585-1588, Jun. 1988.
Russell et al., Infection and Immunity, vol. 56, No. 10, "Streptococcus mutans gtfA Gene Specifies Sucrose Phosphorylase," pp. 2763-2765 (1988).
James et al., Nucleic Acids Research, vol. 16, No. 21, "Nucleotide sequence of the gtfA gene from S. mutans GS-5," p. 10398 (1988).
Ferretti et al., Infection and Immunity, vol. 56, No. 6, "Sequence Analysis of the Glucosyltransferase A Gene (gtfA) from Streptococcus mutans Ingbritt," pp. 1585-1588 (1988).

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