Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2007-10-30
2007-10-30
Cochrane Carlson, Karen (Department: 1656)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S320100, C800S295000, C530S350000
Reexamination Certificate
active
10479638
ABSTRACT:
The present invention relates to the fields of molecular biology and plant biology. Specifically, the invention is directed to the methods for expressing spider silk proteins in plants and the synthesis and purification of spider silk proteins therefrom.
REFERENCES:
patent: 5245012 (1993-09-01), Lombari et al.
patent: 6608242 (2003-08-01), Yang
Guerette et al. 1996; Silk properties determined by gland-specific expression of a spider fibroin gene family. Science 272:112-115.
Scheller, J. et al. “Production of spider silk proteins in tobacco and potato”; Nature Biotechnology, 19: 573-577 (2001).
Austin, S., et al., “Production and field performance of trasngenic alfalfa (Medicago sativa L.) expressing alpha-amylase and manganese-dependent lignin peroxidase”, Euphytica, 85:381-393 (1995).
Bell, A.L., et al., “Changes in Fine Structure During Silk Protein Production in the Ampullate Gland of the SpiderAraneus sericatus”, J. of Cell Biol, 42:284-295, (1969).
Brown, D.C.W., et al., “Role of genetic background in somatic embryogenesis in Medicago”, Plant Cell Tissue Organ Culture 4: 111-122 (1985).
Gil, J., et al., “In Vivo Regulation of Protein Synthesis by Phosphorylation of the α Subunit of Wheat Eukaryotic Initiation Factor 2”, Biochemistry 39: 7521-7530, (2000).
Chang, L-Y, et al., “Specific in vitro phosphorylation of plant eIF2α by eukaryotic eIF2α kinases”, Plant Molecular Biology, vol. 41: 363-370, (1999).
Gil, J., et al., “In Vivo Regulation of the dsRNA-Dependent Protein Kinase PKR by the Cellular Glycoprotein P67”, Biochemistry, 39: 16016-16025, (2000).
Hinman, M.B., et al., “Isolation of a Clone Encoding a Second Dragline Silk Fibroin Nephila clavipes Dragline Silk is a Two-Protein Fiber”, J. of Biol. Chem., vol. 267:19320-19324 (1992).
Huang, S., et al., “Overexpression of 20-Oxidase Confers a Gibberellin—Overproduction Phenotype in Arabidopsis”, Plant Physiol. 118: 773-781, (1998).
Iizuka, E., “The physico-chemical properties of silk fibers and the fiber spinning process”, Experientia, 39: 449-454, (1983).
Lucas, F., “Spiders and their silks”, Discovery 25:20-26 (1964).
Stauffer, S.L., et al., “Comparison of Physical Properties of Three Silks From Nephila clavipes and Araneus gemmoides”, J. of Arachnology, 22:5-11 (1994).
Thiel, B.R., et al., “Physical and Chemical Microstructure of Spider Dragline: A Study by Analytical Transmission Electron Microscopy”, Biopolymers, vol. 34:1089-1097 (1994).
Vollrath, F., “Spider Webs and Silks”, Scientific American Mar. 1992 70-76.
Willcox, P.J., et al., “Evidence of a Cholesteric Liquid Crystalline Phase in Natural Silk Spinning Processes”, Macromolecules, 29: 5106-5110 (1996).
Work, R.W., “Dimensions, Birefringences, and Force-Elongation Behavior of Major and Minor Ampullate Silk Fibers from Orb-Web-Spinning Spiders—The Effects of Wetting on These Properties”, Textile Research Journal, Oct. 1977 p. 650-662.
Xu, M., et al., “Structure of a protein superfiber: Spider dragline silk”, Proc. Natl. Acad. Sci. U.S.A., 87: 7120-7124, (1990).
Scheller, J., et al., “Purification of spider silk-elastin from transgenic plants and application for human chondrocyte proliferation”, Transgenic Research, 13: 51-57 (2004).
Menassa, R., et al., “Spider dragline silk proteins in transgenic tobacco leaves: accumulation and field production”, Plant Biotechnology Journal 2: 431-438 (2004).
Parkhe, A.D., et al., “Structural Studies of Spider Silk Proteins in the Fiber”, Journal of Molecular Recognition, 10: 1-6 (1997).
Horsch, R.B., et al., “A Simple and General Method for Transferring Genes into Plants”, Science, 227: 1229-1231 (1985).
Gosline, J.M., et al., “Spider silk as rubber”, Nature, 309:551-551 (1984).
Abstract, “Plants made to silk”, TRENDS in Genetics 17:442-443, News and Comments, Aug. 2001.
Fischer, E., “About Spider Silk”, Hoppe-Seyler's Zeitschrift fur physiologische Chemie, 53:126-139 (1907).
Kovoor,P.J., “Étude Histochimique et Cytologique des Glands Séricigènes de Quelques Argiopidae”, Annales des Sciences Naturelles, 12:1-40 (1972).
Priuzian, E.S., et al., “Construction of Synthetic Genes for Analogs of Spider Silk Spidroin 1 and Their Expression in Tobacco Plants”, Mol. Biol. (Mosk) 37(4):654-662 (2003).
Micallef, M.C., et al., “Improvement of Transgenic Alfalfa by Backcrossing”, In Vitro Cell Dev. Biol.—Plant 31:187-192, (Oct. 1995).
Gosline, J.M., et al., “The structure and properties of spider silk”, Evdeavour, 10(1):37-43 (1986).
Lewis Randolph V.
Roth Don A.
Carlson Karen Cochrane
Dann Dorfman Herrell & Skillman P.C.
Rigaut Kathleen D.
University of Wyoming
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