Expression of recombinant polypeptides with improved purificatio

Details Expression of recombinant polypeptides with improved purificatio Expression of recombinant polypeptides with improved purificatio

1992-11-12

1994-06-21

Hill, Jr., Robert J.

Chemistry: natural resins or derivatives; peptides or proteins;

Proteins, i.e., more than 100 amino acid residues

435 697, 4352523, 4353201, 530369, 536 234, C07K 1300

Patent

active

053229304

ABSTRACT:
An improved method for expressing peptides as fusion proteins, uses a carrier for a heterologous peptide to provide a fusion protein having a high pI. The high isoelectric point facilitates separation of the fusion protein from all other host cell proteins, and separation of the carrier from the peptide after cleavage.

REFERENCES:
patent: 4431739 (1984-02-01), Riggs
patent: 4532207 (1985-07-01), Brewer et al.
patent: 4743679 (1988-05-01), Cohen et al.
patent: 4764504 (1988-08-01), Johnson et al.
patent: 4880911 (1989-11-01), Brewer et al.
patent: 4897348 (1990-01-01), Johnson et al.
patent: 4987070 (1991-06-01), Magota et al.
Bailey et al., J. Indust. Microbiol. (1987) 2:47-52.
Sung et al., Proc. Natl. Acad. Sci. (1986) 83:561-565.
Cockle et al., "Protein Purification: Micro to Macro" (1987) Alan R. Liss, Inc., pp. 375-381.
Sassenfeld et al., Bio/Technology (Jan. 1984) pp. 76-81.

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