Expression of recombinant polypeptides with improved purificatio

Details Expression of recombinant polypeptides with improved purificatio Expression of recombinant polypeptides with improved purificatio

1990-08-07

1993-04-13

Draper, Garnette D.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 4352523, 4353201, 530350, 530412, C12N 1562, C12N 1512

Patent

active

052022390

ABSTRACT:
An improved method for expressing peptides as fusion proteins, uses a carrier for a heterologous peptide to provide a fusion protein having a high pI. The high isoelectric point facilitates separation of the fusion protein from all other host cell proteins, and separation of the carrier from the peptide after cleavage.

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Bailey et al., J. Indust. Microbiol. (1987) 2:47-52.
Sung et al., Proc. Natl. Acad. Sci. (1986) 83:561-565.
Cockle et al., "Protein Purification: Micro to Macro" (1987) Alan R. Liss, Inc., pp. 375-381.
Sassenfeld et al., Bio/Technology (Jan. 1984) pp. 76-81.

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