Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1995-06-07
1998-10-27
Carlson, Karen
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 4352542, 536235, 536 241, 530385, C12P 2106, C12N 1500, C12N 114, C07K 14805
Patent
active
058276939
ABSTRACT:
The invention is directed to a substantially pure mammalian globin chain or heme-binding fragment thereof. The invention is further directed to recombinant DNA vectors capable of expressing at least one globin chain or substantially homologous variant thereof in yeast. The invention also relates to methods for expressing at least one globin chain or substantially homologous variant thereof in yeast. Expressed alpha-like globin and beta-like globin chains or variants thereof may be combined with a source of heme to produce hemoglobin or a substantially homologous variant thereof. Additionally, expressed gamma-globin chains may be combined with a source of heme to produce hemoglobin or a substantially homologous variant thereof. The invention also relates to methods for expressing hemoglobin or variants thereof in yeast where the heme is produced by the yeast and ligated to globins to form hemoglobin in vivo. The hemoglobin produced by the methods of the present invention may be used in applications requiring a physiological oxygen carrier such as in blood substitute solutions and as in plasma expanders or in applications requiring a physiological oxygen carrier.
REFERENCES:
patent: 5049493 (1991-09-01), Khosia et al.
patent: 5523215 (1996-06-01), Cousens et al.
Motwani et al., 1996, "Production, purification, and characterization of recombinant human hemoglobin rainier expressed in Saccharomyces cerevisiae", Protein Expression and Purification 8:447-455.
J.R. Shaeffer, 1988, "ATP-dependent proteolysis of hemoglobin .alpha. chains in .beta.-thalassemic hemolysates is ubiquitin-dependent", J. of Biol. Chem. 263:13663-13669.
L. Stryer, 1975, "Oxygen-transporting proteins: Myoglobin and hemoglobin", Biochemistry W.H. Freeman & Co. New York Chap.7 pp. 143-174.
Vierstra & Sullivan, 1988, "Hemin inhibits ubiquitin-dependent proteolysis in both a higher plant and yeast", Biochem. 27:3290-3295.
Braverman & Lester, 1981, "Evidence for increased proteolysis in intact .beta. thalassemia erythroid cells", Hemoglobin 5:549-564.
Clements et al., 1989, "Expression and activity of a gene encoding rat cytochrome c in the yeast Saccharomyces cerevisae", Gene 83:1-14.
Datar et al., 1993, "Process economics of animal cell and bacterial fermentations: A case study analysis of tissue plasminogen activator", BioTechnology 11:349-357.
Edman et al., 1981, "Synthesis of hepatitis B surface and core antigens in E. coli", Nature 291:503-506.
Errede et al., 1981, "Studies on transposable elements in yeast I. ROAM mutations causing increased expression of yeast genes: Their activation by signals directed toward conjugation functions and their formation by insertion of Tyl repetitive elements", Cold Spring Harbor Symposia on Quantitative Biology 45:593-607.
Errede et al., 1984, "Identification of regulatory regions within the Tyl transposable element that regulate Iso-2-Cytochrome c production in the CYC7-H2 yeast mutant", Molecular and Cellular Biology 4:1393-1401.
Hallewell et al., 1987, "Amino terminal acetylation of authentic human Cu,Zn superoxide dismutase produced in yeast", BioTechnology 5:363.
Kingsman et al., 1990, "High-efficiency yeast expression vectors based on the promoter of the phosphoglycerate kinase gene", Methods in Enzymology 185:329-341.
Kohno et al., 1990, "Refolding of recombinant proteins", Methods in Enzymology 185:187-195.
Rosenberg et al., 1990, "Glyceraldehyde-3-phosphate dehydrogenase-derived expression cassettes for constitutive synthesis of heterologous proteins", Methods in Enzymology 185:341-351.
Shinar & Rachilewitz, 1990, "Oxidative denaturation of red blood cells in thalassemia", Semin. Hamatol. 27:70-82.
Valenzuela et al., 1982, "Synthesis and assembly of hepatitis B virus surface antigen particles in yeast", Nature 298:347-350.
Wickramasinghe & Hughes, 1984, "Globin chain precipitation, deranged iron metabolism and dyserythropoiesis in some thalassemia syndromes", Haematologia 17:35-56.
Wickramasinghe et al., 1984, "The fate of excess .beta.-globin chains within erythropoietic cells in .alpha.-thalassemia 2 trait, x-thalassemia 1 trait, haemoglobin H disease and haemoglobin Q-H disease: An electron microscope study", Br. J. Haematol. 56:473-482.
Wickramasinghe et al., 1986, "Studies of erythropoietic cells in heterozygotes and homozygotes for haemoglobin Constant Spring and in heterozygotes for both haemoglobin Constant Spring and .alpha.-thalassemia 1 trait: Extent of globin chain precipitation and cell cycle sitribution", Clin. Lab. Haematol. 8:187-198.
J.A. Titus, 1983, Plasmid DNA in colorless filamentous gliding bacteria, Arch. Microbiol. 134:38-44.
Looker et al., 1992, A human recombinant haemoglobin designed for use as a blood substitute, Nature 356: 258-260.
Hoffman et al., 1990, Expression of fully functional tetrameric human hemoglobin in Escherichia coli, Proc. Natl. Acad. Sci. USA 87: 8521-8525.
Jhiang et al., 1989, The structures of the gene encoding chain c of the hemoglobin of the earthworm, Lumbricus terrestris, J. Biol Chem. 264(32): 19003-19008. Arg!: a new variant with analogous properties, Hemoglobin 11(5): 435-452; Adams et al., (1988) Chem. Abs. 108: 545, abst. 129475.
Nagai et al., 1988, Refolding and crystallographic studies of eukaryotic proteins produced in Escherichia coli, Biochem. Soc. Trans. 16: 108-110.
Scarpulla et al., 1986, Functional expression of Rat cytochrome C in Saccharomyces cerevisiae, Proc. Natl. Acad. Sci. USA 83: 6352-6356.
Nagai et al., 1985, Oxygen binding properties of human mutant hemoglobins synthesized in Escherichia coli, Proc. Natl. Acad. Sci. USA 82: 7252-7255.
Dickerson et al., 1983, Hemoglobin: structure, function, evolution, and pathology; Benjamin/Cummings, Menlo Park, CA, pp. 146-158.
Erhart et al., 1983, The presence of a defective LEU2 gene on 2.mu. DNA recombinant plasmids of Saccharomyces cerevisiae is responsible for curing and high copy number, J. Bact. 156 (2): 625-635.
Gibb, E.A., 1981, Increased subunit association of a new super stable variant hemoglobin motown, Clin. Res. 29(4):795A.
Lauer et al., 1980, The chromosomal arrangement of human .alpha.-like globin genes: sequence homology and .alpha. globin gene deletions, Cell 20: 119-130.
Altay et al., 1976, Hemoglobin Hacettepe of .alpha..sub.2 .beta..sub.2 .sup.127(H5)Gln.fwdarw.Glu , Biochem. Biophys. Acta. 434: 1-3.
Oshino et al., 1973, Studies on yeast hemoglobin. The properties of yeast hemoglobin and its physiological function in the cell, Eur. J. Biochem. 35: 23-33.
Oshino et al., 1973, Purification and molecular properties of yeast hemoglobin, Eur. J. Biochem. 39: 581-590.
Oshino et al., 1972, Yeast hemoglobin-reductase complex, Biochem. Biophys. Res. Comm. 46(3): 1055-1060.
U.S. Patent No. 5,028,588, issued Jul. 2, 1991, by Hoffman and Nagai.
Wagenbach et al., 1991, Synthesis of wild type and mutant human hemoglobins in Saccharomyces cerevisiae, Biotechnology 9:57-61.
European Patent Publication No. 0 402 300 A2, dated Dec. 12, 1990.
PCT International Publication No. WO 88/09179, dated Dec. 1, 1988.
PCT International Publication No. WO 90/13645, dated Nov. 15, 1990.
PCT International Publication No. WO 89/03883, dated May 5, 1989.
Greaves et al., 1990, A transgenic mouse model of sickle cell disorder, Nature 343:183-185.
Hanscombe et al., 1989, High-level, erythroid-specific expression of the human .alpha.-globin gene in transgenic mice and the production of human hemoglobin in murine erythrocytes, Genes & Dev. 3:1572-1581.
Dobson et al., 1989, Identification of the latency-associated transcript promoter by expression of rabbit beta-globin mRNA in mouse sensory nerve ganglia latently infected with a recombinant herpes simplex virus, J. Virol. 63:3844-3851.
Springer and Sligar, 1987, High-level expression of sperm whale myoglobin in Escherichia coli, Proc. Natl. Acad. Sci. USA 84:8961-8965.
Miller et al., 1988, Design of retrovirus vectors for transfer and expression of the human .beta.-Globin Gene, J. Virol. 62:4337-4345.
Walder, 1988, The use of recombinant hemoglobin as a blood substitute, Biotechnology 88:357-362.
Brinigar et al., Expression
Bajwa Wajeeh
Bonaventura Joseph
De Angelo Joseph
Motwani Nalini M.
Apex Bioscience, Inc.
Carlson Karen
LandOfFree
Expression of recombinant hemoglobin and hemoglobin variants in does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Expression of recombinant hemoglobin and hemoglobin variants in , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression of recombinant hemoglobin and hemoglobin variants in will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1613086