Expression of proteins on bacterial surface

Details Expression of proteins on bacterial surface Expression of proteins on bacterial surface

1991-11-15

1994-09-20

Hill, Jr., Robert J.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 698, 435 711, 435 712, 4351721, 4352521, 4352523, 43525233, 4353201, 536 234, C12P 2106, C12P 2104, C12N 1500, C12N 120

Patent

active

053488679

ABSTRACT:
The invention relates to a method for producing stable, surface-expressed polypeptides from recombinant gram-negative bacterial cell hosts. A tripartite chimetic gene and its related recombinant vector include separate DNA sequences for directing or targeting and translocating a desired gene product from a cell periplasm to the external cell surface. A wide range of polypeptides may be efficiently surface expressed, including .beta.-lactamase and alkaline phosphatase. Full enzyme activity is maintained and the proteins remain anchored to the bacterial outer membrane surface.

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