Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Patent
1997-03-03
2000-03-14
Smith, Lynette F.
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
A01H 500
Patent
active
060375272
ABSTRACT:
A plant expression vector is constructed to cause the expression of an amino-terminal portion of the Bacillus thuringiensis delta-endotoxin gene in plant cells and the vector is used to create transgenic plants expressing the toxin. A truncated form of the toxin is used, with carboxy-terminal prolines added for stability. A translational enhancer sequence derived from the untranslated leader sequence from the mRNA of the coat protein gene of alfalfa mosaic virus coat protein gene is placed between a promoter and the toxin gene to increase translational efficiency. The transgenic plants produced are toxic to Lepidopteran pests and can transmit that trait to their progeny by normal Mendelian inheritance.
REFERENCES:
patent: 5608142 (1997-03-01), Barton et al.
Koziel MG, et al. "Optimizing expression of transgenes with an emphasis on post-transcriptional events." Plant Mol. Biol. 32: 393-405, 1996.
Barton Kenneth A.
Umbeck Paul F.
Ball Timothy K.
Hoerner Dennis R.
Monsanto Company
Nelson Amy J.
Smith Lynette F.
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