Expression of MDR-related gene in yeast cell

Details Expression of MDR-related gene in yeast cell Expression of MDR-related gene in yeast cell

1992-11-18

1995-03-21

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving antigen-antibody binding, specific binding protein...

43525421, 4353171, C12Q 102, C12N 119

Patent

active

053994833

ABSTRACT:
A yeast host which can express P-glycoprotein, i.e., the product of MDR-related gene, in the cell membrane in the same state as observed in multidrug resistant cells produced by connecting the MDR-related gene which carries multidrug resistance to a yeast expression vector and transforming the yeast with said recombinant vector; a cell membrane fraction containing a substantial amount of P-glycoprotein produced by said yeast and a process for the preparation thereof; and a recombinant vector for expressing the MDR-related gene in a yeast host.

REFERENCES:
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Kelley et al., "Isolation and characterization of a .lambda.polA tranducing phage", Proc. Natl. Acad. Sci. USA, vol. 74, No. 12, pp. 5632-5636, Dec. 1977, Genetics; see p. 5635, second paragraph, left column.
Fujita et al., "Biosynthesis of the Torpedo California Acetylcholine Receptor .alpha. Subunit in Yeast", Science, vol. 231, pp. 1284-1287; see p. 1286, left upper col., lines 5-10, (1986).
Safa et al., "Identification of the Multidrug Resistance-related Membrane Glycoprotein as an Acceptor for Calcium Channel Blockers", JBC 262(16):7884-7888 (Jun. 1987).
Udda et al., "The mdrl gene, responsible for multidrug resistance, codes for P-glycoprotein", Biochem. Biophys. Res. 141(3):956-962 (Dec. 1986).
Gros et al., "Mammalian Multidrug Resistance Gene: Complete cDNA sequence Indicates Strong Homology to Bacterial Transport Proteins", Cell 47:371-380 (Nov. 1986).

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