Expression of mature proteinase 2A, the partial purification the

Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

4353201, 4352523, 4351723, 435219, 435212, 536 2372, 536 232, 935 9, 935 14, 935 22, 935 29, 935 72, 935 73, C12N 121, C12N 1570

Patent

active

055590250

ABSTRACT:
The invention relates to the expression of mature HRV proteinase 2A, the partial purification thereof and the preparation of substrates having an inhibitory effect.

REFERENCES:
patent: 5149783 (1992-09-01), Sommergruber et al.
Stedman's Medical Dictionary 25th Edition, 1990, Hensyl et al.(eds.), Williams & Wilkins., Baltimore, MD. p. 792.
Agol, V.I., "Structure, translation, and replication of picornaviral genomes", Prog. Med. Virol.26:119-157 (1980).
Butterworth, B. E., "A comparison of the virus-specific polypeptides of encephalomyocarditis virus, human rhinovirus", Virology 56:439-453 (1973).
Callahan, P. L. et al., "Molecular cloning and complete sequence determination of RNA genome of human rhinovirus type 14", Proc. Natl. Acad. Sci. USA 82:732-736 (Feb. 1985).
Duechler, M. et al., "Evolutionary relationships within the human rhinovirus genus: comparison of sterotypes 89, 2, and 14", Proc. Natl. Acad. Sci. USA 84:2605-2609 (May 1987).
Hanecek, R. et al., "Proteolytic processing of poliovirus polypeptides: antibodies to polypeptides P3-7c inhibit cleavage at glutamine-glycine pairs", Proc. Natl. Acad. Sci. USA 79:3973-3977 (Jul. 1982).
Hughes, P. J. et al., "The nucleotide sequence of human rhinovirus 1B: molecular relatonships within the rhinovirus genus", J. Gen. Virol.69:49-58 (1988).
Konig, H. and Rosenwirth, B., "Purification and partial characterization of poliovirus protease 2A by means of a functional assay", J. Virol. 62(4):1243-1250 (Apr. 1988).
Kowalski, H. et al., "Cleavage site between VP1 and P2A of human rhinovirus is different in stereotypes 2 and 14", J. Gen. Virol. 68:3197-3200 (1987).
Krausslich, H. G. and Wimmer, E., "Viral proteinases", Ann. Rev. Biochem. 57:701-754 (1988).
Krausslich, H. G. et al., "Poliovirus proteinase 2A induces cleavage of eucaryotic initiation factor 4F polypeptide p220", J. Virol. 61(9):2711-2718 (Sep. 1987).
Lee, C. K. and Wimmer, E., "Proteolytic processing of poliovirus polyprotein: elimination of 2A.sup.pro -mediated, alternative cleavage of polypeptide 3CD by in Vitro mutagenesis", Virol.166:405-414 (1988).
Lloyd, R. E. et al., "Cleavage of the cap binding protein complex polypeptide p220 is not effected by the second poliovirus protease 2A", Virol. 150:299-303 (1986).
Lloyd, R. E. et al., "restriction of translation of capped mRNA in vitro as a model for poliovirus-induced inhibition of host cell protein synthesis: relationship to p220 cleavage", J. Virol. 61(8):2480-2488 (Aug. 1987).
McLean, C. and Rueckert, R. R., "Picornaviral gene order: comparison of a rhinovirus with a cardiovirus", J. Virol. 11(2):341-344 (Feb. 1973).
McLean, C. et al., "Evidence of ambiguous processing and selective degradation in the noncapsid proteins of rhinovirus 1A", J. Virol. 19(3):903-914 (Sep. 1976).
Nicklin, M. J. H., "Poliovirus polypeptide precursors: expression in vitro and processing by exogenous 3C and 2A proteinases", Proc. Natl. Acad. Sci. USA, 84:4002-4006 (Jun. 1987).
Palmenberg, A. C. , "Chapter 13-Sequence alignments of picornaviral capsid proteins", Molecular Aspects of Picornavirus Infection and Detection, Semler and Ehrenfeld, eds., Washington, D.C., American Society for Microbiology, 211-241 (1989).
Palmenberg, A. C., "Picornaviral processing: some new ideas", j. Cell.Biochem. 33:191-198 (1987).
Putnak, J. R. and Phillips, B. A., "Picornaviral structure and assembly", Microbiol. Rev. 45(2):287-315 (Jun. 1981).
Skern, T. et al., "Human rhinovirus 2: complete nucleotide sequence and proteolytic processing signals in the capsid protein region", Nucleic Acids Res. 13(6):2111-2126 (1985).
Sommergruber, W. et al., "Polypeptide 2A of human rhinovirus type 2: identification as a protease and characterization by mutational analysis", Virology 169:68-77 (1989).
Stanway et al., "the nucleotide sequence of poliovirus type 3 leon 12 a.sub.1 b: comparison with poliovirus type 1", Nucl. Acids Res. 11(16): 5629-5643 (1983).
Stanway, G. et al., "The complete nucleotide sequence of a common cold virus: human rhinovirus 14", Nucleic Acids Res. 12(20):7859-7875 (1984.
Stanway, G., "Structure, function, and evolution of picornaviruses", J. Gen. Virol. 71:2483-2501 (1990).
Billich et al., Synthetic Peptides as Substrates and Inhibitors of Human Immune Deficiency Virus-1 Protease, Journal of Biological Chemistry 263(34):17905-17908 (1988).
English abstract of Japanese Patent document JP-A-56 086 142 (Reference AL1), Derwent abstract No. 81-63212D.
Clonetech Catalog, 1991-1992, p. 132, Palo Alto, CA.

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Expression of mature proteinase 2A, the partial purification the does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Expression of mature proteinase 2A, the partial purification the, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression of mature proteinase 2A, the partial purification the will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-1928081

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.