Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1991-07-31
1994-03-01
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4353201, 4351723, 4352402, C12N 510, C12P 2102
Patent
active
052906868
ABSTRACT:
The present invention relates to baculovirus-expressed influenza antigens, in particular, to the influenza A membrane protein, M2, expressed from Autographa Californica nuclear polyhedrosis virus (AcNPV). The present invention further relates to a method to increase the yield of baculovirus-expressed M2 proteins in host cells by culturing the recombinant baculovirus infected host cells with an amantadine-like drug. Other aspect of the present invention relate to the use of baculovirus-expressed M2 proteins in reproducible and routine assays for the seradiagnosis of influenza A virus infections as an alternative to the more burdensome complement fixation and hemagglutination tests.
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Sugrue et al, Virology 180:617-624 (1991).
Lamb, R. A., et al., "Influenza Virus M.sub.2 Protein Is an Integral Membrane Protein Expressed on the Infected-Cell Surface," Cell 40:627-633 (1985).
Schmaljohn, C. S., et al., "Baculovirus Expression of the Small Genome Segment of Hantaan Virus and Potential Use of the Expressed Nucleocapsid Protein as a Diagnostic Antigen," J. Gen Virol. 69:777-786 (1988).
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Zebedee, S. L., et al., "Characterization of the Influenza Virus M.sub.2 Integral Membrane Protein and Expression at the Infected-Cell Surface from Cloned cDNA," J.Virol. 56(2):502-511 (Nov. 1985).
Black Renee
Kendal Alan P.
Rota Paul A.
Guzo David
Schwartz Richard A.
The United States of America as represented by the Department of
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