Expression of human serum albumin in Pichia pastoris

Details Expression of human serum albumin in Pichia pastoris Expression of human serum albumin in Pichia pastoris

1995-05-03

1998-01-13

Robinson, Douglas W.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435 696, 435 698, 4351721, 4352555, 4353201, 935 22, 935 28, 935 33, 935 37, 935 66, 935 69, C12P 2106, C12N 119, C12N 1500, C12N 1563

Patent

active

057078289

ABSTRACT:
The present invention is directed to expression cassettes comprising a 5' regulatory region from at least one of the Pichia pastoris AOXI gene, p40 gene, DASI gene or HIS4 gene, operably linked to an HSA structural gene including the HSA signal sequence. The HSA structural gene has a translational start codon within 0 to 11 deoxyribonucleotides from the 5' end of the HSA structural gene and is operably linked to a 3' termination sequence. Further, the adenine and thymine content of the intervening deoxyribonucleotides is in the range from about 55 to about 64%. The expression cassette may be an autonomously replicating vector or an integrative vector. Other embodiments of the present invention include Pichia pastoris strains transformed with the HSA expression cassettes and processes for secretion of HSA using the expression cassettes.

REFERENCES:
patent: 4775622 (1988-10-01), Hitzeman
patent: 4808537 (1989-02-01), Stroman et al.
patent: 4837148 (1989-06-01), Cregg
patent: 4855231 (1989-08-01), Stroman et al.
patent: 4882279 (1989-11-01), Cregg
patent: 4885242 (1989-12-01), Cregg
patent: 4895800 (1990-01-01), Tschopp et al.
Bitter et al., "Expresion of Heterologous Genes in Saccharomyces cerevisiae from Vectors Utilizing the Glyceraldehyde-3-Phosphate Dehydrogenase Gene Promoter," Gene, 32: 268-274 (Dec. 1984).
Digan et al., "Secretion of Heterologous Proteins from the Methylotrophic Yeast Pichia pastoris," Developments in Industrial Microbiology, 29(supp 3): 59-65 (1988).
A. Dugaiczyk, "Nucleotide Sequence and the Encoded Amino Acids of Human Serum Albumin mRNA," Proc. Natl. Acad. Sci. U.S.A., 79, 71-75 (Jan. 1982).
Ellis et al., "Isolation of Alcohol Oxidase and Two Other Methanol Regulatable Genes from the Yeast Pichia pastoria," Mol. Cell. Biol., 5(5): 1111-1121.
T. Etcheverry, "Regulation of the Chelatin Promoter During the Expression of Human Serum Albumin or Yeast Phoshpoglycerate Kinase in Yeast," Bio/Technology, 4: 726-730 (Aug. 1986).
M. Latta, "Synthesis and Purification of Mature Human Serum Albumin from E. coli," Nuc. Acid. Res., 9(22): 6103-6114 (Nov. 1981).
T. Nobuhira et al., "Amino Acid Substitution on Genetic Variants of Human Serum Albumin," Proc. Natl. Acad. Sci. U.S.A., 84: 4413-4417 (Jul. 1987).
J. Peters, Albumins: Structure, Biosynthesis, Function, pp. 11-17 (1977).
C. Saunders, "Secretion of Human Serum Albumin from Bacillus subtilis," J. Bacteriology, 169(7): 2917-2925 (Jul. 1987).
Sleep et al., "The Secretion of Human Serum Albumin from the Yeast Saccharomyces cerevisiae Using Five Different Leader Sequences," Bio/Technology, 8: 42-46 (Jan. 1990).
Chemical Abstracts, vol. 107, No. 1, Jul. 6, 1987, p. 533, Abstract No. 5669u.
Abstract of JP 60248181, published Dec. 7, 1985.
Abstract of EP 123544, published Jul. 16, 1986.
Abstract of EP 91527, published Oct. 19, 1983.
Abstract of EP 20123, published Dec. 10, 1980.

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