Expression of human immunodeficiency virus (HIV) reverse transcr

Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for...

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435974, 930221, 935 33, 935 38, 935 40, 935 47, 935 73, C12N 900, C12N 1549

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052565548

ABSTRACT:
This invention describes pHRT25, a plasmid containing a modified pol gene of the Human Immunodeficiency Virus Type 1 (HIV-1), formerly HTLV-III, under control of an inducible trp promoter. Methods of expressing reverse transcriptase activity using pHRT25 in E. coli are described.

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