Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1988-07-18
1992-12-15
Schwartz, Ricahrd A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435202, 4352523, 43525231, 4353201, 4351723, 536 27, 935 29, 935 27, C12N 928, C12N 121, C12N 1575, C12N 2100
Patent
active
051716739
ABSTRACT:
A vector for high-level expression of a heterologous gene in Bacillus, including a DNA sequence which encodes all or part of the structural gene and contains the promoter and ribosome binding site of a Bacillus coagulans amylase gene; within or immediately downstream of the structural gene is a site for insertion of heterologous DNA; when inserted, the heterologous DNA is in the same translational reading frame as the amylase gene; the heterologous DNA thus is under control of the B. coagulans amylase gene regulatory elements and expressed at high levels. A signal-encoding sequence may be inserted independent of, or along with, the heterologous DNA leading to authentic secretion of the heterologous protein.
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Hannett Nancy M.
Pero Janice
Rudolph Cathy F.
Rufo, Jr. Gerald A.
Sloma Alan
BioTechnica International, Inc.
LeGuyader John L.
Schwartz Ricahrd A.
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