Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1984-07-17
1986-10-21
Hazel, Blondel
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
4351723, 435317, 536 27, 935 27, 935 28, 935 12, C12P 2100, C12N 1500, C12N 100, C07H 2104
Patent
active
046185780
ABSTRACT:
Methods and compositions are provided for the efficient production in yeast of polypeptides which are immunologically cross-reactive with glycoprotein D of the herpes simplex virus. Synthetic DNA fragments encoding for a portion of the glycoprotein, and the naturally-occurring gene encoding for the glycoprotein and portions thereof, are expressed on plasmids in yeast. Secretion may be provided by including a secretory leader and signal processing sequence derived from the .alpha.-factor gene. Alternatively, the genes may be expressed intracellularly under the transcriptional control of a promoter derived from a gene in the yeast glycolytic pathway.
E. coli strains HB101 containing plasmids pYHS109 and pYHS118 were deposited at the American Type Culture Collection on July 11, 1984, and granted accession nos. 39762 and 39763, respectively.
REFERENCES:
patent: 4317811 (1982-03-01), Bertland et al.
patent: 4374127 (1983-02-01), Larson et al.
J. Hilfenhaus et al., (1981) 17th Intl. Cong. Herpes Virus of Man and Animal, 52:321-331.
Watson et al., (1982) Science, 218:381-382.
Roizman et al., (1982) Devel. Biol. Standard, 52:287-304.
Nahmias et al., (1979) Disease-a-Month, 25:5-49.
Berman et al., (1983) Science, 222:524-527.
Weis et al., (1983) Nature, 302:72-74.
Cohen et al., (1984) J. Virol., 49:102-108.
Laskey et al., (1984) Biotechnology, pp. 527-532.
Burke Rae L.
Urdea Mickey S.
Valenzuela Pablo D. T.
Chiron Corporation
Hazel Blondel
LandOfFree
Expression of glycoprotein D of herpes simplex virus does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Expression of glycoprotein D of herpes simplex virus, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression of glycoprotein D of herpes simplex virus will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1289815