Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1997-05-12
1999-06-22
Ulm, John
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4352523, 4353201, 530350, 536 234, C07K 1900, C12N 1562
Patent
active
059142545
ABSTRACT:
The invention is directed to the use of a fusion partner that does not contain a leader sequence, as a means to increase the solubility and activity of recombinant polypeptides by facilitating the expression of fusion proteins, which are then transported out of the cytoplasm. The invention includes a nucleic acid encoding a fusion polypeptide comprising a mature interleukin-1-like polypeptide or a leader-deleted-translocating polypeptide, and a polypeptide of interest; as well as host cells comprising such nucleic acids, and fusion proteins so encoded. The invention also encompasses methods of using such nucleic acids to produce recombinant fusion polypeptides, mature polypeptides of interest, and purified compositions thereof.
REFERENCES:
patent: 4595658 (1986-06-01), Zinder et al.
patent: 4801536 (1989-01-01), Stahl et al.
patent: 5084384 (1992-01-01), Wong et al.
patent: 5143830 (1992-09-01), Holland et al.
patent: 5563046 (1996-10-01), Mascarenhas et al.
patent: 5629172 (1997-05-01), Mascarenhas et al.
Bardwell, J.C.A. et al., "Identification of a protein required for disulfide bond formation in vivo" Cell (1991) 67:581-589.
Bayer, M.E., "Response of cell walls of Escherichia coli to a sudden reduction of the environmental osmotic pressure" J. Bacteriol. (Mar. 1967) pp. 1104-1112.
Chang, A.C.Y. et al., "Construction and characterization of amplifiable multicopy DNA cloning vehicles derived from the P15A cryptic miniplasmid" J. Bacteriol. (1978) pp. 1141-1156.
Cooper, D.N.W. et al., "Evidence for export of a muscle lectin from cytosol to extracellular matrix and for a novel secretory mechanism" J. Cell Biol. (1990) 110:1681-1691.
di Guan, C. et al., "Vectors that facilitate the expression and purification of foreign peptides in Escherichia coli by fusion to maltose-binding protein" Gene (1988) 67:21-30.
Eisenberg, S.P. et al., "Interleukin 1 receptor antagonist is a member of the interleukin 1 gene family: Evolution of a cytokine control mechanism" Proc. Natl. Acad. Sci. USA (1991) 88:5232-5236.
Eisenberg, S.P. et al., "Primary structure and functional expression from complementary DNA of a human interleukin-1 receptor antagonist" Nature (1990) 343:341-346.
Engler, D.A. et al., "Cloning of authentic human epidermal growth factor as a bacterial secretory protein and its initial structure-function analysis by site-directed mutagenesis" J. Biol. Chem. (1988) 263(25):12384-12390.
Furman, T.C. et al., "Recombinant human insulin-like growth factor II expressed in Escherichia coli" Bio/Technology (Oct. 1987) 5:1047-1051.
Goeddel, D.V. et al., "Expression in Escherichia coli of chemically synthesized genes for human insulin" Proc. Natl. Acad. Sci. USA (1979) 76(1):106-110.
Habazetti, J. et al., "Structure of hisactophilin is similar to interleukin-1J and fibroblast growth factor" Nature (1992) 359:855-858.
Holland, IB et al., "Haemolysin secretion from E. Coli" Biochimie (1990) 72:131-141.
Hsiung, H.M. et al., "High level expression, efficient secretion and folding of human growth hormone in Escherichia coli" Bio/Technology (1986) 4:991-995.
Jacobson, G.R. et al., "Properties of a major protein released from Escherichia coli" Biochem. (1976) 15(11):2298-2302.
Johnson, K.S. et al., "Vaccination against ovine cysticercosis using a defined recombinant antigen" Nature (1989) 338:585-587.
Joseph-Liauzun, E. et al., "Human recombinant interleukin-1J isolated from Escherichia coli by simple osmotic shock" Gene (1990) 86:291-295.
Kamitani, S. et al., "Identification and characterization of an Escherichia coli gene required for the formation of correctly folded alkaline phosphatase, a periplasmic enzyme" Embo J. (1992) 11:57-62.
Kato, C. et al., "Construction of an excretion vector and extracellular production of human growth hormone from Escherichia coli" Gene (1987) 54:197-202.
Kellenberger, E., "The `bayer bridges` confronted with results from improved electron microscopy methods" Mol. Microbiol. (1990) 4(5):697-705.
LaVallie, E.R. et al., "A thioredoxin gene fusion expression system that circumvents inclusion body formation in the E. coli cytoplasm" Bio/Technology (1993) 11:187-193.
Lunn, C.A. et al., "Localization of thioredoxin from Escherichia coli in an osmotically sensitive compartment" J. Biol. Chem. (1982) 257(19):11424-11430.
McDonald, N.Q. et al., "A structural superfamily of growth factors containing a cystine knot motif" Cell (1993) 73:421-424.
Miller, H.I. et al., "Cloning and expression of a yeast ubiquitin-protein cleaving activity in Escherichia coli" Bio/Technology (1989) 7:698-704.
Muesch, A. et al., "A novel pathway for secretory proteins?" TIBS 15 (Mar. 1990) pp. 86-88.
Nagahari, K., et al., "Secretion into the culture medium of a foreign gene product from Escherichia coli: Use of the ompF gene for secretion of J-endorphin" EMBO J. (1985) 4(13a):3589-3592.
Peek, J.A. et al., "Characterization of a periplasmic thiol: disulfide interchange protein required for the functional maturation of secreted virulence factors of Vibrio cholerae" Proc. Natl. Acad. Sc. USA (1992) 89:6210-6214.
Priestle, J.P. et al., "Crystallographic refinement of interleukin 1J at 2.0 .ANG. resolution" Proc. Natl. Acad. Sci. USA (1989) 86:9667-9671.
Pugsley, A., "The complete general secretory pathway in gram-negative bacteria" Microbiol. Rev. (Mar. 1993) pp. 50-108.
Rubartelli, A. et al., "A novel secretory pathway for interleukin-1J, a protein lacking a signal sequence" Embo J. (1990) 9(5):1503-1510.
Rubartelli, A. et al., "Secretion of thioredoxin by normal and neoplastic cells through a leaderless secretory pathway" J. Biol. Chem. (1992) 267(34):24161-24164.
Schoner, R.G. et al., "Isolation and purification of protein granules from Escherichia coli cells overproducing bovine growth hormone" Bio/Technology (Feb 1985) pp. 151-154.
Schultz, M-F. et al., "Increased expression in Escherichia coli of a synthetic gene encoding human somatomedin C after gene duplication and fusion" J. Bacteriol. (Dec. 1987) pp. 5385-5392.
Singer, I.I. et al., "Interleukin 1J is localized in the cytoplasmic ground substance but is largely absent from the golgi apparatus and plasma membranes of stimulated human monocytes" J. Exp. Med. (1988) 167:389-407.
Squires, C.H. et al., "Production and characterization of human basic fibroblast growth factor from Escherichia coli" J. Biol. Chem. (1988) 263(31):16297-16302.
Studier, F.W. et al., "Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes" J. Mol. Biol. (1986) 189:113-130.
Sutcliffe, J.G., "Nucleotide sequence of the ampicillin resistance gene of Escherichia coli plasmid pBR322" Proc. Natl. Acad. Sci. USA (1978) 75(8):3737-3741.
Tobias, J. W., et al., "Cloning and functional analysis of ubiquitin-specific protease gene UPB1 of saccharomyces cerevisiae" J. Biol. Chem. (1991) 266(18):12021-12028.
von Heijne, G. "A new method for predicting signal sequence cleavage sites" Nucleic Acids Res. (1986) 14(11):4683-4690.
Williams, D.C. et al., "Cytoplasmic inclusion bodies in Escherichia coli producing biosynthetic human insulin proteins" Science (1992) 215:687-689.
Young, P. et al., "Modularity of protein function: chimeric interleukin 1Js containing specific protease inhibitor loops retain function of both molecules" Biochem. (1993) 32:5327-5331.
Zhang, J. et al., "Three-dimensional structure of human basic fibroblast growth factor, a structural homolog of interleukin 1J" Proc. Natl. Acad. Sci. USA (1991) 88:3446-3450.
Zhu, X. et al., "Three-dimensional structures of acidic and basic fibroblast growth factors" Science (1991) 251:90-94.
Swimmer et al. Phage display of ricin B chain and its single binding domains: System for screening galactose-binding mutants. Proc. Natl. Acad. Sci. 89:3756-3763 May 1992.
Hussain et al. Expression of ricin B chain in Escherichia coli. FEBS Letts. 244(2):383-387 Feb. 1989.
Uhlen et al. Gene Fusions for Purpose of Expression: An Introduction. Meth. In Enzymol. 185:129-143 Jan. 1990.
Transue et al. Structure of benzyl T-antigen disaccharide bound to Amaranthus caudatus agglutinin. Nature: structural biolog
Cohen Pedro A.
Mascarenhas Desmond
Nguyen Kimyen B.
Olsen David R.
Olson Pamela S.
Celtrix Pharmaceuticals Inc.
Ulm John
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