Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound other than saccharide containing a...
Patent
1994-08-02
1997-05-13
Ulm, John
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound other than saccharide containing a...
435 691, 4352523, 4353201, 530315, 536 234, C12N 119, C12N 1562, C07K 1400
Patent
active
056291728
ABSTRACT:
The invention is directed to the use of a fusion partner that does not contain a leader sequence, as a means to increase the solubility and activity of recombinant polypeptides by facilitating the expression of fusion proteins, which are then transported out of the cytoplasm. The invention includes a nucleic acid encoding a fusion polypeptide comprising a mature interleukin-1-like polypeptide or a leader-deleted-translocating polypeptide, and a polypeptide of interest; as well as host cells comprising such nucleic acids, and fusion proteins so encoded. The invention also encompasses methods of using such nucleic acids to produce recombinant fusion polypeptides, mature polypeptides of interest, and purified compositions thereof.
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Cohen Pedro A.
Mascarenhas Desmond
Olsen David R.
Olson Pamela S.
Zhang Yang
Celtrix Pharmaceuticals Inc.
Ulm John
LandOfFree
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