Expression of foreign genes using a replicating polyprotein prod

Details Expression of foreign genes using a replicating polyprotein prod Expression of foreign genes using a replicating polyprotein prod

1993-11-01

1996-02-13

Fox, David T.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Using tissue cell culture to make a protein or polypeptide

435 691, 435 694, 435 6952, 435 696, 435 911, 435 9133, 4351723, 4353201, C12N 701, C12N 1501, C12P 2100

Patent

active

054910765

ABSTRACT:
The present invention provides an expression vector adapted for expressing heterologous proteins in plants susceptible to a polyprotein-producing plant virus. The vector utilizes the unique ability of viral polyprotein proteases to cleave heterologous proteins from viral polyproteins. Also provided is a method for expressing heterologous proteins in plants using these unique expression vectors.

REFERENCES:
patent: 4874702 (1989-10-01), Fiers et al.
patent: 4874703 (1989-10-01), Jaskunas
patent: 5162601 (1992-11-01), Slightom
V. V. Dolja, et al. "Spontaneous Mutagenesis of a Plant Potyvirus Genome After Insertion of a Foreign Gene" J. Virology 67:5968-5975, No. 10 (1993).
V. V. Dolja, et al. "Tagging of Plant Potyvirus Replication and Movement of Insertion of .beta.-Glucuronidase into the Viral Polyprotein" Proc. Natl. Acad. Sci. USA 89:10208-10212 (1992).
J. Donson, et al. "Systemic Expression of a Bacterial Gene by a Tobacco Mosaic Virus-Based Vector" Proc. Natl. Acad. Sci. USA 88:7204-7208 (1991).

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