Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1986-12-16
1988-11-15
Weimar, Elizabeth
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
4351723, 4352402, 435320, 935 11, 935 32, 935 48, 935 60, 935 70, 536 27, 530384, C12N 1500, C12P 2102, C07H 1512, C07K 1300
Patent
active
047849509
ABSTRACT:
Methods are disclosed for producing proteins having biological activity for blood coagulation mediated by Factor VIIa. The proteins are produced by mammalian host cells which have been stably transfected with a DNA construct containing a nucleotide sequence which codes at least partially for either Factor VII. The nucleotide sequence comprises a first nucleotide sequence encoding a calcium binding domain, joined to a second nucleotide sequence positioned downstream of the first sequence. In particular, the first nucleotide sequence may be derived from a genomic clone or cDNA clone of Factor VII. The second sequence encodes a catalytic domain for the serine protease activity of Factor VIIA. The joined sequences code for proteins having substantially the same biological activity for blood coagulation as Factor VIIa.
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Berkner Kathleen L.
Busby Sharon J.
Gray Charles L.
Hagen Frederick S.
Insley Margaret Y.
Weimar Elizabeth
ZymoGenetics Inc.
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