Expression of exogenous polypeptides and polypeptide products in

Details Expression of exogenous polypeptides and polypeptide products in Expression of exogenous polypeptides and polypeptide products in

1990-09-24

1993-03-30

Martinell, James

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

935 37, C12P 2100

Patent

active

051983486

ABSTRACT:
Novel yeast cell transformation vectors are manufactured and employed in securing expression of exogenous polypeptides in yeast cells. Vectors include promoter/regulator DNA sequences of yeast glyceraldehyde-3-phosphate dehydrogenase gene origins. In an illustrative preferred embodiment, novel immunologically active hepatitis B surface antigen (HBsAg) preparations are isolated from yeast cells transformed with plasmid A.T.C.C. 40053. These HBsAg preparations of yeast origin may be incorporated into vaccine compositions useful in developing immunological responses protective against infection by hepatitis B virus.

REFERENCES:
Hitzeman et al; Nature 293: 717 (1981).

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