Expression of exogenous polypeptides and polypeptide products in

Chemistry: molecular biology and microbiology – Vector – per se

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435 693, 435 711, 4351723, 435255, 435256, 935 34, 935 37, 935 69, C12N 1500, C12N 116, C12N 118, C12P 2100

Patent

active

049770926

ABSTRACT:
Novel yeast cell transformation vectors are manufactured and employed in securing expression of exogenous polypeptides in yeast cells. Vectors include promoter/regulator DNA sequences of yeast glyceraldehyde-3-phosphate dehydrogenase gene origins. In an illustrative preferred embodiment, novel immunologically active hepatitis B surface antigen (HBsAg) preparations are isolated from yeast cells transformed with plasmid A.T.C.C. 40053. These HBsAg preparations of yeast origin may be incorporated into vaccine compositions useful in developing immunological responses protective against infection by hepatitis B virus.

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