Expression of eukaryotic peptides in plant plastids

Details Expression of eukaryotic peptides in plant plastids Expression of eukaryotic peptides in plant plastids

2007-08-21

2007-08-21

Fox, David T. (Department: 1638)

Multicellular living organisms and unmodified parts thereof and

Method of introducing a polynucleotide molecule into or...

C800S288000, C435S069100, C435S069400, C435S069700, C435S419000, C536S023510, C536S023600, C536S023720, C536S024100

Reexamination Certificate

active

10917774

ABSTRACT:
Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences are expressed to produce eukaryotic peptide in said plastid.

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