Chemistry: molecular biology and microbiology – Animal cell – per se ; composition thereof; process of... – Primate cell – per se
Patent
1997-01-29
2000-10-31
Wityshyn, Michael G.
Chemistry: molecular biology and microbiology
Animal cell, per se ; composition thereof; process of...
Primate cell, per se
435325, 435366, 435378, C12N 500, C12N 502, C12N 508
Patent
active
061401196
ABSTRACT:
A method is described for culturing morphologically and antigenically distinguishable types of normal human breast epithelial cells (HBEC) derived from reduction mammoplasty. Type I HBEC showed luminal and stem cell characteristics i.e. the ability to form budding/ductal structures when cultured on MATRIGEL and expressed a variant ER (.about.48 Kd) which has a deletion in the DNA binding domain (exon 2). In contrast, Type II HBEC with basal epithelial phenotype are ER-negative when cultured on MATRIGEL. Simian virus 40 (SV40) transformed Type I and Type II HBEC lines also expressed the variant ER. Tumors formed in athymic nude mice by in vitro transformed tumorigenic Type I cell lines, however, expressed a high level of wild type ER which was undetectable in these cells grown in vitro before and after tumor formation. Thus, there appears to be a differentional ER mRNA splicing between the in vitro and in vivo milieu.
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Chang Chia-Cheng
Trosko James
Kerr Janet M.
Michigan State University
Wityshyn Michael G.
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