Expression of DNA sequences encoding a thermally stable cytosine

Details Expression of DNA sequences encoding a thermally stable cytosine Expression of DNA sequences encoding a thermally stable cytosine

1990-06-01

1994-08-16

Weimar, Elizabeth C.

Chemistry: molecular biology and microbiology

Enzyme , proenzyme; compositions thereof; process for...

Hydrolase

4352402, 4352523, 4353201, 536 232, 536 2374, C12N 500, C12N 120, C12N 1500, C07H 1512

Patent

active

053386787

ABSTRACT:
Thermally stable cytosine deaminase (CDase), and the gene coding therefor, is disclosed as well as methods of isolating, purifying, and recombinantly producing the same. The thermally stable CDase can be isolated from Saccharomyces cerevisiae. The yeast isolated enzyme has a molecular weight of approximately 32 kDa, as determined by gel filtration chromatography, and is composed of two subunits, each with a molecular weight of about 17 kDa. Thermally stable yeast CDase so purified shows no significant sequence homology with other known sequenced proteins.

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