Expression of DNA sequences derived from nocardioform microorgan

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

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435 712, 435190, 4352532, 43525235, 4352531, 4353201, 435486, 536 232, 536 241, 536 237, C12P 2106, C12P 2104, C12N 1553, C12N 1576

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060227036

ABSTRACT:
A cloning vector contains a DNA sequence derived from a Nocardioform microorganism, and is capable of expressing the DNA sequence in a host microorganism. Recombinant cholesterol oxidase is produced by a host microbial cell transformed with a cloning vector containing cholesterol oxidase encoding DNA derived from a Nocardioform microorganism. A method for determining cholesterol oxidase in a sample of fluid from a human includes contacting the sample with the Nocardioform microorganism derived recombinant cholesterol oxidase.

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