Expression of Cry3B insecticidal protein in plants

Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C800S279000, C435S320100, C435S418000, C536S023710, C424S093200

Reexamination Certificate

active

06943281

ABSTRACT:
The present invention discloses methods and compositions comprising a group of novel expression cassettes which provide significantly improved levels of accumulation of Coleopteran inhibitory Cry3B and Cry3B variant amino acid sequences when these are expressed in plants. The preferred embodiments of the invention provide at least up to ten fold higher levels of insect controlling protein relative to the highest levels obtained using prior compositions. In particular, transgenic maize expressing higher levels of a protein designed to exhibit increased toxicity toward Coleopteran pests deliver superior levels of insect protection and are less likely to sponsor development of populations of target insects that are resistant to the insecticidally active protein.

REFERENCES:
patent: 5024837 (1991-06-01), Donovan et al.
patent: 5378625 (1995-01-01), Donovan et al.
patent: 5424412 (1995-06-01), Brown et al.
patent: 5500365 (1996-03-01), Fischhoff et al.
patent: 5659123 (1997-08-01), VanRie et al.
patent: 5689052 (1997-11-01), Brown et al.
patent: 6023013 (2000-02-01), English et al.
patent: WO 91/14778 (1991-10-01), None
patent: WO 97/13402 (1997-04-01), None
patent: WO 98/23641 (1998-06-01), None
patent: WO 99/31248 (1999-06-01), None
Lazar et al, 1988, Mol. Cell. Biol. 8:1247-1252.
Hill et al, 1998, Biochem. Biophys. Res. Comm. 244:573-577.
Iannacone et al, 1997, Plant Mol. Biol. 34:484-496.
Crickmore et al, 1998, Microbiol. Mol. Biol. Rev. 62:807-813.
Arpaia et al., Production of transgenic eggplant (Solanum melongena L.) resistant to Colorado Potato Beetle (Leptinotarsa decemlineala Say),Theor. Appl. Genet.95:329-334 (1997).
Chen et al., Transfer and Transcriptional Expression of Coleopteran cryIIIB Endotoxin Gene ofBacillus thuringiensisin Eggplant,J. Amer. Soc. Hort. Sci.120:921-927 (1995).
Diehn et al, Problems that can limit the expression of foreign genes in plants: Lessons to be learned from B.t. toxin genes,Genetic Engineering18:83-99 (1996).
Lamppa et al., Structure and Developmental Regulation of a Wheat Gene Encoding the Major Chlorophyll a/b-Binding Polypeptide,Molecular and Cellular Biology5:1370-1378 (1985).
McGaughey et al., Managing insect resistance toBacillus thuringiensistoxins,Science258:1451-1455 (1992).
Murray et al., Codon usage in plant genes,Nucleic Acids Research17:477-498 (1989).
Roush, Managing pests and their resistance toBacillus thuringiensis: Can transgenic crops be better than sprays?,Biocontrol Science and Technology4:501-516 (1994).
Sutton et al., Synthetic cryIIIA gene fromBacillus thuringiensisimproved for high expression in plants,Transgenic Research1:228-236 (1992).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Expression of Cry3B insecticidal protein in plants does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Expression of Cry3B insecticidal protein in plants, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression of Cry3B insecticidal protein in plants will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-3374118

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.