Expression of core-glycosylated HCV envelope proteins in yeast

Drug – bio-affecting and body treating compositions – Antigen – epitope – or other immunospecific immunoeffector – Amino acid sequence disclosed in whole or in part; or...

Reexamination Certificate

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C424S228100, C424S193100, C424S194100, C424S196110, C435S005000, C435S069300, C435S235100, C435S238000, C435S239000, C530S404000, C530S412000, C530S415000, C530S422000

Reexamination Certificate

active

07048930

ABSTRACT:
The present invention relates to the general field of recombinant protein expression, purification of recombinant proteins, diagnosis of HCV infection, prophylactic treatment against HCV infection and to the prognosing/monitoring of the clinical efficiency of treatment of an individual with chronic hepatitis, or the prognosing/monitoring of the natural disease. In particular, the present invention relates to the use of yeast, i.e.HansenulaorSaccharomycesglycosylation minus strains, for the efficient expression of HCV envelope proteins that are core-glycosylated, purification methods for these proteins, and the use in various applications, such as the use in diagnosis, prophylaxis or therapy of HCV envelope proteins purified according to the present invention.

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