Expression of biologically active PDGF analogs in yeast

Details Expression of biologically active PDGF analogs in yeast Expression of biologically active PDGF analogs in yeast

1984-10-12

1988-09-06

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Enzymatic production of a protein or polypeptide

435 70, 435 91, 4351723, 435255, 435320, 935 13, 935 28, 935 37, C12P 2100, C12P 1934, C12N 1500, C12N 500

Patent

active

047693282

ABSTRACT:
Biologically active PDGF analogs expressed in yeast are disclosed. The analogs are produced by yeast strains transformed with an extrachromosomal element composed of a strong transcriptional promoter directing the expression of a gene which encodes a protein having substantially the same biological activity as PDGF. Suitable genes include the v-sis gene or a derivative of the v-sis gene of simian sarcoma virus or portions thereof, or the human cDNA gene for PDGF or portions thereof. A secretory signal sequence may be provided upstream of the gene, enabling secretion of the gene product from the yeast host cell. Mitogenic activity is one of the biological activities possessed by these PDGF analogs, making them useful in promoting the growth of mammalian cells.

REFERENCES:
patent: 4350687 (1982-09-01), Lipton et al.
Devare et al. (1984) Cell, vol. 36, pp. 43-49.
Robbins et al. (1983) Nature, vol. 305, pp. 605-608.
Kurjan et al. (1982) Cell, vol. 30, pp. 933-943.
Davis et al. (1980) Nature 283, pp. 433-488.
Alber et al. (1982) Journal of Molecular and Applied Genetics, vol. 1, pp. 419-434.
Brake, A. et al., Aug. 1984, PNAS 81:4642.
Wang, J. et al., Sep. 1984, J.B.C. 259:10645.
Clarke, M. et al., Mar. 1984, Nature 308:464.
Johnnson, A. et al., May 1984, EMBOJ, 3:921.
Owen, A. et al., Jul. 1984, Science 225:54.
Edens, L. et al., Jun. 1984, Cell 37:629.
Production of Platelet-Derived Growth Factor-Like Molecules and Reduced Expression of Platelet-Derived Growth Factor Receptors Accompany Transformation by a Wide Spectrum of Agents, D. F. Bowen-Pop et al., Proc. Natl. Acad. Sci., vol. 81, Apr. 1984, pp. 2396-2400.
Expression of the PDGF-Related Transforming Protein of Simian Sarcoma Virsu in E. coli, S. G. Devare et al., CELL, vol. 36, pp. 43-49, Jan., 1984.
The C-Sis Gene Encodes a Precursor of the B Chain of Platelet-Derived Growth Factor, A. Johnsson et al., the EMBO Journal, vol. 3, No. 5, pp. 921-928, 1984.
Simian Sarcoma Virus Onc Gene, V-Sis, is Derived from the Gene (or Genes) Encoding a Platelet-Derived Growth Factor, R. F. Doolittle et al., Science, vol. 221, pp. 275-277, Jul. 15, 1983.
Structural and Immunological Similarities Between Simian Sarcoma Virus Gene Product)(s) and Human Platelet-Derived Growth Factor, K. C. Robbins et al., Nature, vol. 305, Oct. 13, 1983, pp. 605-608.
Secretion of Foreign Proteins from Saccharomyces Cerevisiae Directed by Alpha Factor Gene Fusions, C. A. Bitter, et al., Proc. Natl. Acad. Sci. U.S.A., vol. 81, pp. 5330-5334, Sep. 1984.
Expression of a Platelet-Derived Growth Factor-Like Protein in Simian Sarcoma Virus Transformed Cells, T. F. Deuel et al., Science, vol. 221, pp. 1348-1350.
Transforming Potential of Human C-Sis Nucleotide Sequences Encoding Platelet Derived Growth Factor, S. F. Josephs et al., Science, vol. 225, Aug. 10, 1984, pp. 636-639.
Simian Sarcoma Virus-Transformed Cells Secrete a Mitogen Identical to Platelet-Derived Growth Factor, A. J. Owen et al., Science, vol. 225, Jul. 6, 1984, pp. 54-56.
A V-Sis Oncogene Protein Produced in Bacteria Competes for Platelet-Derived Growth Factor Binding to its Receptor, J. Y. J. Wang et al., Journal of Biological Chemistry, vol. 259, No. 17, 9/10/84, pp. 10645-10648.
Structure of a Yeast Pheromone Gene (MF.alpha.): a Putative .alpha.-Factor Precursor Contains Four Tandem Copies of Mature .alpha.-Factor, J. Kurjan et al., Cell, vol. 30, pp. 933-943, Oct., 1982.

Affiliated with

Also associated with

Rating

Expression of biologically active PDGF analogs in yeast does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Expression of biologically active PDGF analogs in yeast, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression of biologically active PDGF analogs in yeast will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1606651