Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2005-04-26
2005-04-26
Leffers, Gerry (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S069200, C435S320100, C435S483000, C435S255100, C435S255500, C435S255600, C435S254200, C530S355000
Reexamination Certificate
active
06884602
ABSTRACT:
This invention involves a process for selecting and producing eukaryotic alkaline phosphatase in yeast. Yeast cells are subjected to multiple transformations using a vector comprising a first resistance marker gene and the alkaline phosphatase gene. Those strains that grow in media containing the first resistance marker are further transformed using a vector comprising a second selection marker gene and the alkaline phosphatase gene. Transformants that grow in media containing the second selection marker are selected for expressing the eukaryotic alkaline phosphatase.
REFERENCES:
patent: 0399455 (1990-11-01), None
patent: 0700997 (1996-03-01), None
patent: 0955369 (1999-11-01), None
patent: 2200118 (1988-07-01), None
patent: WO 9904014 (1999-01-01), None
patent: WO 0056900 (2000-09-01), None
patent: WO 0166693 (2001-09-01), None
Reinhard, Beck et al, “ Expression of Human Placental Alkaline Phosphatase inEscherichia coli”, Protein Expression And Purification 5, 192-197 (1994).
Batard, Yannick et al., “Increasing Expression of P450 and P450-Reductase Proteins from Monocots in Heterologous Systems,” Archives of Biochemistry and Biophysics, vol. 379, No. 1, Jul. 1, pp. 161-169, 2000.
Clare, Jeffrey J. et al., “Production of mouse epidermal growth factor in yeast: high-level secretion usingPichia pastorisstrains containing multiple gene copies,” Gene, 105 (1991) 205-212.
Li, Hong et al., “The Relation between Codon Using, Base Correlation and Gene Expression Level inEscherichia coliand Yeast,” J. Theor. Biol. (1996) 181, 111-124.
Linder, Stefan et al., “Expression of Reticulomyxa filosa tubulins inPichia pastoris: regulation of tubulin pools,” FEBS Letters 417 (1997) 33-37.
Sreekrishna, Koti et al., “Stategies for optimal synthesis and secretion of heterologous proteins in the methylotrophic yeastPichia pastoris,” Gene 190 (1997) 55-62.
Zhao, X et al., “ Synonymous condon usage inPichia pastoris,” Database Public Medline 'Online! NCBI; PMID: 11059269, May 2000.
Joel Berger, et al., “Expression of Active, Membrane-Bound Human Placental Alkaline Phosphatase by Transfected Simian Cells”, Proc. Natl. Acad. Sci., USA vol. 84, pp. 4885-4889, Jul. 1987, Biochemistry.
James M. Cregg, et al., “Pichia Pastorisas a Host System for Transformations” Molecular and Cellular Biology, Dec. 1985, p. 3376-3385, vol. 5, No. 12.
T.R. Davis, et al., “Baculovirus Expression of Alkaline Phosphatase as a Reporter Gene for Evaluation of Production, Gycosylation and Secretion”Biotechnology, vol. 10, Oct. 1992 (p. 1148-1150).
Harry Harris, “ The Human Alkaline Phosphatases: Whate We Know and What We Don't Know” Clinica Chemica Acta, 186 (1989) 133-150.
Thomas Manes, et al. “Genetic Complexity, Structure, and Characterization of Highly Active Bovine Intestinal Alkaline Phosphatases”, The Journal of Biological Chemistry, vol. 273, No. 36, Issue of Sep. 4, pp. 23353-23360, 1998.
Robert B. McComb, et al., (1979) in Alkaline Phosinhatases, Plenum Press, New York and London (158pgs).
Jose Luis Millan, “Oncodevelopmental Expression and Structure of Alkaline Phosphatase Genes” Anticancer Research 8: 995-1004 (1988).
Robert K. Scopes, “Protein Purification” PA0007/002/003, Springer-Verlag, New York, Heidelberg Berlin, London, Paris, Tokyo (9pgs).
Heige Weissig, et al. “Cloning and Expression of the Bovine Intestinal Alkaline Phosphatase Gene: Biochemical Characterization of the Recombinant Enzyme” Biochem J. (1993) 290, 503-508, Printed in Breat Britain.
Bommarius Bettina
Eckstein Hellmut
Geipel Frank
Glaser Stephan
Hoelke Werner
Amick Marilyn
Leffers Gerry
Marvich Maria
Roche Diagnostics Operations Inc.
Roche Diagnostics Operations Inc.
LandOfFree
Expression of alkaline phosphatase in yeast does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Expression of alkaline phosphatase in yeast, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression of alkaline phosphatase in yeast will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3374384