Expression of a human insulin precursor in P. pastoris

Details Expression of a human insulin precursor in P. pastoris Expression of a human insulin precursor in P. pastoris

2006-08-15

2006-08-15

Guzo, David (Department: 1636)

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Fungi

C435S254100, C435S254110, C435S254200

Reexamination Certificate

active

07091032

ABSTRACT:
Methylotrophic recombinant yeast strain producing human insulin precursor, the strain comprising, in its genome, a copy of a first DNA construction and a second DNA construction, wherein the constructions are capably of directing the expression and secretion of human insulin precursor of the formula B(1-30)-Y1-Y2-A(1-21), wherein Y1 is lysine or arginine; Y2 is lysine or arginine; B(1-30) is the B peptide of the human insulin; and B(1-21) is the A peptide of human insulin, and wherein the strain is yeastPichia Pastoris. DNA constructions and method for obtaining the strain are also provided.

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