Expression monitoring by hybridization to high density oligonucl

Details Expression monitoring by hybridization to high density oligonucl Expression monitoring by hybridization to high density oligonucl

1995-09-15

2000-03-21

Zitomer, Stephanie W.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

536 231, 536 243, 536 2431, 536 2432, 536 2433, C12Q 168, C07H 2104, C07H 2102

Patent

active

060401389

ABSTRACT:
This invention provides methods of monitoring the expression levels of a multiplicity of genes. The methods involve hybridizing a nucleic acid sample to a high density array of oligonucleotide probes where the high density array contains oligonucleotide probes complementary to subsequences of target nucleic acids in the nucleic acid sample. In one embodiment, the method involves providing a pool of target nucleic acids comprising RNA transcripts of one or more target genes, or nucleic acids derived from the RNA transcripts, hybridizing said pool of nucleic acids to an array of oligonucleotide probes immobilized on surface, where the array comprising more than 100 different oligonucleotides and each different oligonucleotide is localized in a predetermined region of the surface, the density of the different oligonucleotides is greater than about 60 different oligonucleotides per 1 cm.sup.2, and the olignucleotide probes are complementary to the RNA transcripts or nucleic acids derived from the RNA transcripts; and quantifying the hybridized nucleic acids in the array.

REFERENCES:
patent: 4981783 (1991-01-01), Augenlicht
patent: 5002867 (1991-03-01), Macevicz
patent: 5143854 (1992-09-01), Pirrung et al.
patent: 5173260 (1992-12-01), Zander et al.
patent: 5202231 (1993-04-01), Drmanac et al.
patent: 5422241 (1995-06-01), Goldrick et al.
patent: 5571639 (1996-11-01), Hubbell et al.
patent: 5690894 (1997-11-01), Pinkel et al.
patent: 5700637 (1997-12-01), Southern et al.
Fornace et al., 1988, Proc. Natl. Science. USA 85:8800-8804.
Fornance et al., 1984, Explt. Cell Res. 1082:61-74.
Hollbrook et al, 1991, The New Biologist 3:825-833.
Nature Biotechnology, vol. 14, No. 13, Dec. 1996, Nature Publ. Co., New York, US, pp. 1675-1680, XP002022521 D.J. Lockhart et al.: "Expression monitoring by hybridization to high-density oligonucleotide arrays".
Proc. Natl. Acad. Sci. U.S.A. (1996), 93(20), 10614-10619 CODEN: PNASA6; ISSN: 0027-8424, Oct. 1, 1996, XP002022507 Schena, Mark et al.: "Parallel human genome analysis: microarray-based expression monitoring of 1000 genes".
Science, vol. 274, Oct. 25, 1996, AAAS, Washington, DC, US, pp. 610-614, XP002022508 M. Chee et al.: "Accessing genetic information with high-density DNA arrays".
Velculescu, V.E., et al, 1995. Serial Analysis of Gene Expression. Science 270:484-487.
Gress, T.M. et al, 1992. Hybridization Fingerprinting of High-Density cDNA-library Arrays cDNA Pools Derived from Whole Tissues, Mammalian Genome 3:609-619.
Nguyen, C. et al, 1995. Differential Gene Expression in the Murine Thymus Assayed by Quantative Hybridization of Arrayed cDNA Clones. Genomics 29-207-216.
Zhao, N. et al, 1995. High-Density cDNA Filter Analysis A Novel Approach for Large-Scale Quantitative Analysis of Gene Expression. Gene 156:207-213.
Takahashi N. et al., 1995. High-Density cDNA Filter Analysis of the Expression Profiles of the Genes Preferentially Expressed in Human Brain. Gene 164:219-227.
Schena, M. et al, 1995. Quantitative Monitoring of Gene Expression Patterns with a Complementary DNA Microarray. Science 270:467-470.
Meier-Ewert, S. et al, 1993. An Automated Approach to Generating Expressed Sequence Catalogs. Nature 361:6410: 375-376.
Eggers et al., BioFeature, 17:516 (1994).
Kallioniemi et al., Science, 258:818 (1992).
Broude et al., Proc. Natl. Acad. Sci. USA, 91:3072 (1994).
Fodor et al., Research Article, 767 (1991) .music-flat. page numbers?.
Lee et al., Analytical Biochemistry, 206:206 (1992).
Lennon et al., Perspectives TIG October, 7:314 (1991).
Lipshutz et al., BioTechniques 19:442 (1995).
Pevzner et al., Journal of Biomolecular Structures & Dynamics,, 9:399 (1991).
Schena et al., Science, 270:467 (1995).
Southern et al., Genomics 13:1008 (1992).
Southern et al., Nucleic Acids Research, 22:1368 (1994).
Stimpson et al., Proc.Natl.Acad. Sci. USA, 92:6379 (1995).
Lennon et al. TIG 7: 314-317, 1991.
Hoheisel TIG 10,No. 3, 1994.

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