Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical
Reexamination Certificate
2006-05-09
2006-05-09
McKelvey, Terry (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Preparing compound containing saccharide radical
C435S455000, C435S468000
Reexamination Certificate
active
07041483
ABSTRACT:
The present invention provides a method for modulating expression of a genetic sequence by introducing, creating or deleting one or more pseudo-translation initiation sites in the nucleotide sequence of an mRNA, upstream of the authentic translation initiation site of an open reading frame. Expression of the genetic sequence can be further modulated by introducing, creating or removing Kozac or Kozac-like sequences proximal to the pseudo-translation initiation site(s). Moreover, expression can be manipulated by the introduction, creation or removal of a termination signal prior to the authentic translation initiation site or after this site but in a different reading frame relative to the reading frame determined by the authentic translation initiation site. Nucleic acid molecules useful for practicing the present methods are also provided. The present invention further provides a method for detecting a disease condition associated with a particular level of expression of a gene or other genetic sequence.
REFERENCES:
patent: 5262316 (1993-11-01), Engler et al.
patent: 5648267 (1997-07-01), Reff
patent: 6051409 (2000-04-01), Hansen et al.
patent: 6306636 (2001-10-01), Haselkorn et al.
Deffaud, Clarence, et al. (2000) “Characterization of an Internal Ribosomal Entry Segment in the 5' Leader of Murine Lukemia VirusenvRNA”Journal of Virology 74(2): 846-850.
Fiaschi, Tania, et al. (2000) “The Inhibitory Effect of the 5' Untranslated Region of Muscle Acylphosphatase mRNA on Protein Expression is Relieved During Cell Differentiation”,FEBS Letters 473: 42-46.
Geballe, Adam P., et al. (1988) “Translational Control of Cytomegalovirus Gene Expression is Mediated by Upstream AUG Codons”,Journal of Virology 62(9); 3334-3340.
Sella, Osnat et al. (1999) “Differentiation-Induced Internal Translation of c-sismRNA: Analysis of thecisElements and Their Differentiation-Linked Binding to the hnRNP C Protein”,Molecular and Cellular Biology 19(8) : 5429-5440.
Sarrazin, Sandrine, et al. (2000) “Negative and Translation Termination-Dependent Positive Control of FLI-1 Protein Synthesis by Conserved Overlapping 5' Upstream Open Reading Frames in Fli-1 m RNA”,Molecular and Cellular Biology 20(9) : 2959-2969.
Cazzola, Mario, et al. (2000) “Translational Pathophysiology: A Novel Molecular Mechanism of Human Disease”,Blood 95(11): 3280-3288.
Rimbault, B., et al. (2000) “Identification of the Initiation Condon for theatpBGene inChlamydomonasChlotoplasts Excludes Translation of a Precursor Form of the β Subunit of the ATP Synthase”,Mol Gen Genet 264: 486-491; and.
Kozak, Marilyn (2000) “Do the 5' Untranslated Domains of Human cDNAs Challenge the Rules for Initiation of Translation (or is it Versa)?”,Genomics 70: 396-406.
Pain, V.M. (1986), “Initiation of Protein Synthesis in Mammalian Cells”, Biochem. J. 235: 625-637.
Modave, K. (1985, “Eukaryotic Protein Synthesis”, Ann. Rev. Biochem. 54: 1109-1149.
Kozak, M. (1986), “Point Mutations Define a Sequence Flanking the AUG Initiator Codon that Modulates Translation by Eukaryotic Ribosomes”, Cell. 44: 283-292.
Sonenberg, N. (1990), Poliovirus Translation, Curr. Top. Micro. And Imm. 161: 23-47.
Carrington, J.C. and Freed, D.D. (1990), “Cap-Independent Enhancement of Translation by a Plant Potyvirus 5' Nontranslated Region”, J. of Vir. 64 : 1590-1597.
Jackson et al. (1990), “The Novel Mechanism of Initiation of Picornavirus RNA Translation”, TIBS 15: 477-483.
Kinzler et al. (1987), “Identification of an Amplified, Highly Expressed Gene in a Human Glioma”, Science 236: 70-73.
Roberts et al. (1989), “Amplification of the gli Gene in Childhood Sarcomas”, Cancer Res. 49: 5407-5413.
Stein et al. (1999), “GLI Gene Expression in Bone and Soft Tissue Sarcomas of Adult Patients Correlates with Tumor Grade”, Cancer Res. 59: 1890-1895.
Ingham, P.W. (1998), “Transducing Hedgehog: The Story so Far”, EMBO J. 17: 3505:3511.
Johnson, R.L. and Scott, M.P. (1998), “New Players and Puzzles in the Hedgehog Signaling Pathway”, Curr. Opin. Genet. Dev. 8: 450-456.
Ruiz T. Altraba, A. (1999), “The Works of GLI and the Power of Hedgehog”, Nature Cell Biol. 1: 147-148.
Kinzler et al. (1998), The GLI Gene is a Member of the Kruppel Family of Zinc Finger Proteins, Nature 332: 371-374.
Ruppert et al. (1988), “The GLI-Kruppel Family of Human Genes”, Mol. Cell. Biol. 8: 3104-3113.
Walterhouse et al. (1993) “gli, a Zinc Finger Transceiption Factor and Oncogene, is Expressed During Normal Mouse Development”, Dev. Dyn. 196: 91-102.
Hui et al. (1994), “Expression of three Mouse Homologs of the Drosophila Sengment Polarity Gene cubitus interruptis, Gli, Gli-2 and Gli-3, in Ectoderm- and Mesoderm-Derived Tissues Suggests Multiple Roles during Postimplantation Development”, Dev. Biol. 162: 402-413.
Marigo et al. (1996), “Sonic Hedgehog Differentially Regulates Expression of GLI and GLI3 during Limb Development”, Dev. Biol. 180: 273-283.
Lee et al. (1997), “Gli1 is a Target of Sonic Hedgehog that Induces Ventral Neural Tube Development”, Development 124: 2537-2552.
St-Jacques et al. (1998), “Sonic Hedgego Signaling is Essential for hair Development”, Curr. Biol. 8: 1058-1068.
Chiang et al. (1999), “Essential Role for Sonic hedgehog during Hair Follicle Morphogenesis” , Dev. Biol. 205: 1-9.
Gailani et al. (1996), The Role of the Human Homologue of Drosophila Patched in Sporadic Basal Cell Carcinomas, Nat. Genet. 14: 78-81.
Hahn et al. (1996) “Mutations of the Human Homolog of Drosophila Patched in the Nevoid Basal Cell Carcinoma Syndrome”, Cel 85: 841-851.
Johnson et al. (1996), “Human Homolog of patched. a candidate Gene for the Basal Cell Nevus Syndrome”, Science 272: 1668-1671.
Fan et al. (1997), “Induction of Basal Cell Carcinoma Features in Transgenic Human Skin Expressing Sonic Hedgehog”, Nat. Med. 3: 788-792.
Oro et al. (1997), “ Basal Cell Carcinomas in Mice Overexpressing Sonic Hedgehog”; Science 276: 817-821.
Xie et al. (1998), “Activating Smoothened Mutations in Sporadic Basal Cell-Cacinoma”; Nature 391 90-92.
Dahmane et al. (1997), “Activation of the Transcription Factor Gli1 and the Sonic Hedgehog Signalling Pathway in Skin Tumours”, Nature 389: 876-881.
Ruppert et al. (1991 “The Zinc Finger Protein GLI Transforms Primary Cells in Cooperation with Adenovirus E1A”, Mol. Cell. Biol., 11: 1724-1728.
Reifenberger et al. (1998), “Missense Mutations in SMOH in Sporadic Basal Cell Carcinomas of the Skin and Primitive Neuroectodermal Tumors of the Central Nervoius System”, Cancer Res. 58: 1798-1803.
Gnali, et al. (1999), “Gli1 Protein is Expressed in Basal Cell Carcinomas Outer Root Sheath Keratinocytes and a Subpopulation of Mesenchymal Cells in Normal Human Skin”, Invest. Dermatol 113: 595-599.
Motoyama, et al. (1998) “Essential Function of Gli2 and Gli3 in the Formation of Lung, Tachea and Oesophagus”, Nat. Genet., 20: 54-47.
Dai, et al. (1999), “Sonic Hedgehog-induced Activation of the Gli1 Promoter Is Mediated by GLI3”, J. Biol. Chem. 274: 8143-8152.
Ruiz I. Altaba, A. (1999), “Gli Proteins Encode Context-Dependent Positive and Negative Functions” Implications for Development and Disease, Development 126: 3205-3216.
Sasaki, et al. (1999), “Regulation of Gli2 and Gli3 Activates by Amino-Terminal Repression Domain: Implication of Gli2 and Gli3 as Primary Mediators of Shh Signalling”, Development 126: 3915-3924.
Altschul et al. (1997), “Gapped BLAST and PSI-BLAST: A New Generation of Protein Database Search Programs”, Nucl. Acids Res. 25: 3389.
Ausubel et al. “Current Protocols in Molecular Biology”, John Wiley & Sons, Inc.
Rothnagel Joseph Attila
Wang Xue-Qing
McKelvey Terry
Scully Scott Murphy & Presser
The University of Queensland
LandOfFree
Expression modulating sequences does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Expression modulating sequences, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression modulating sequences will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-3602377