Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Enzymatic production of a protein or polypeptide
Patent
1984-04-12
1988-09-06
Hazel, Blondel
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Enzymatic production of a protein or polypeptide
435320, 4351723, 536 27, C12P 2100, C12N 1500, C07H 2104
Patent
active
047693266
ABSTRACT:
The present invention is based upon a general principle of providing specific oligonucleotide segments ("linkers", herein) to be attached in sequence to a cloned DNA coding segment. The linkers of the present invention confer desired functional properties on the expression of the protein coded by the coding sequence. Using linkers of the present invention, the desired protein may be expressed either as a fusion or non-fusion protein. A linker coding for an additional sequence of amino acids may be attached, the sequence being chosen to provide properties exploitable in a simplified purification process. A linker coding for an amino acid sequence of the extended specific cleavage site of a proteolytic enzyme is provided, as well as specific cleavage linkers for simpler specific cleavage sites.
REFERENCES:
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Goeddel et al., Proc. Natl. Acad. Sci., vol. 76, pp. 106-110, 1979.
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Marians et al., Nature, vol. 263, pp. 744-748, 1976.
Heyneker et al., Nature, vol. 263, pp. 748-752, 1976.
Stryer, Lubert; Biochemistry, 1981, W. H. Freeman and Company, New York, pp. 25 & 26 (photocopied).
Hazel Blondel
Murphy Lisabeth Feix
The Regents of the University of California
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