Expression library immunization

Details Expression library immunization Expression library immunization

1997-12-30

1999-11-23

Scheiner, Laurie

Drug, bio-affecting and body treating compositions

Antigen, epitope, or other immunospecific immunoeffector

Amino acid sequence disclosed in whole or in part; or...

4241841, 4241851, 4241881, 4242011, 4242071, 4242081, 4242341, 4242631, 4242641, 4242481, 435325, 435440, 435455, 435489, 530403, 530806, 530825, 530826, 530868, 514 2, A61K 3900, A61K 3921

Patent

active

059895535

ABSTRACT:
A general method for vaccinating against any pathogen is presented. The method utilizes expression library immunization, where an animal is inoculated with an expression library constructed from fragmented genomic DNA of the pathogen. All potential epitopes of the pathogen's proteins are encoded in its DNA, and genetic immunization is used to directly introduce one or more expression library clones to the immune system, producing an immune response to the encoded protein. Inoculation of expression libraries representing portions of the Mycoplasma pulmonis genome was shown to protect mice from subsequent challenge by this natural pathogen. Protection against Listeria.

REFERENCES:
patent: 5451399 (1995-09-01), Gimbrone, Jr. et al.
patent: 5589466 (1996-12-01), Feigner et al.
patent: 5703057 (1997-12-01), Johnston et al.
Davis et al., "DNA-based immunization induces continuous secretion of hepatitis B surface antigen and high levels of circulating antibody," Human Molecular Genetics, 2:(11)1847-1851, 1993.
Eisenbraun et al., "Examination of Parameters Affecting the Elicitation of Humoral Immune Responses by Particle Bombardment-Mediated Genetic Immunization," DNA Cell Biol. 12(9):791-797, 1993.
Fynan et al., "DNA vaccines: Protective Immunizations by parenteral, mucosal and gene-gun inoculations," Proc. Natl. Acad. Sci. USA, 90:11478-11482, 1993.
Kovacsovics-Bankowskiet al., "Efficient major histocompatibility complex class I presentation of exogenous antigen upon phagocytosis by macrophages," Proc. Natl. Acad. Sci. USA, 90:4942-4946, 1993.
Lai et al., "Protection of mice against experimental murine mycoplasmosis by a Mycoplasma pulmonis immunogen in lysogenized Escherichia coli," Vaccine, 12(4):291-298, 1994.
Lowrie et al., "Towards a DNA vaccine against tuberculosis," Vaccine, 12(16):1537-1540, 1994.
Tang et al., "Genetic immunization is a simple method for eliciting an immune response," Nature, 356:152-154, 1992.
Ulmer et al., "Heterologous Protection Against Influenza by Injection of DNA Encoding a Viral Protein," Science, 259:1745-1749, 1993.
Wang et al., "Gene inoculation generates immune responses against human immunodeficiency virus type 1," Proc. Natl. Acad. Sci. USA, 90:4156-4160, 1993.
Xiang et al., "Vaccination with a Plasmid Vector Carrying the Rabies Virus Glycoprotein Gene Induces Protective Immunity against Rabies Virus," Virology, 199:132-140, 1994.
Klein, In: Immunology: The Science of Self-Nonself Discrimination, John Wiley & Sons, New York, pp. 6-9, 660-661, 1982.
Stryer, In: Biochemistry, W.H. Freeman and Co., San Francisco, p 732, 1975.
Soukhanov et al. (Eds.), In: Webster's II. New Riverside University Dictionary, The Houghton Mifflin Co., Boston, MA, p 67, 1984.
Ada, "Strategies for Exploiting the Immune System in the Design of Vaccines," Molec. Immunol., 28(3):225-230, 1991.
Ausebel, Ed., In: Short Protocols in Molecular Biology A Compendium of Methods from Current Protocols in Molecular Biology, 2nd Ed., Harvard Medical School, publication date unknown.
Barry et al., "Production of Monoclonal Antibodies by Genetic Immunization," BioTechniques, 16(4):616-620, 1994.
Coney et al., "Facilitated DNA inoculation induces anti-HIV-1 immunity in vivo," Vaccine, 12(16):1545-1550, 1994.
Conry et al., "Immune Response to a Carcinoembryonic Antigen Polynucleotide Vaccine," Cancer Research, 54:1164-1168, 1994.
Cox et al., "Bovine Herpesvirus 1: Immune Responses in Mice and Cattle Injected with Plasmid DNA," J. Virol., 67(9):5664-5667, 1993.
Barry et al., "Protection against mycoplasm infection using expression-library immunization," Nature, 377:632-635, 1995.
Crystal, "Transfer of genes to humans: Early lessons and obstacles to success," Science, 270:404-409, 1995.
Wang et al., "DNA inoculation induces cross clade anit-HIV-1 responses," Annals of the New York Academy of Sciences, 772:186-197, 1995.
Warner et al., "Retroviral vectors for HIV immunotherapy," Annals of the New York Academy of Sciences, 772:105-116, 1995.
Asubel et al., In: Current Protocols in Molecular Biology, John-Wiley & Sons, New York, pp. 6.0.3-6.0.4, 1988.

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