Expression library immunization

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536 221, 536 231, 536 232, 536 234, 536 235, 536 2351, 536 2372, 536 2374, 536 237, 435325, 4353201, 4351723, 4351721, 435 6, 435 71, 424422, 424423, 424 92, A01N 4304

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057030570

ABSTRACT:
A general method for vaccinating against any pathogen is presented. The method utilizes expression library immunization, where an animal is inoculated with an expression library constructed from fragmented genomic DNA of the pathogen. All potential epitopes of the pathogen's proteins are encoded in its DNA, and genetic immunization is used to directly introduce one or more expression library clones to the immune system, producing an immune response to the encoded protein. Inoculation of expression libraries representing portions of the Mycoplasma pulmonis genome was shown to protect mice from subsequent challenge by this natural pathogen. Protection against Listeria was also obtained using the method.

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