Expression enhancer and use thereof to increase the yield in the

Details Expression enhancer and use thereof to increase the yield in the Expression enhancer and use thereof to increase the yield in the

1993-01-22

1994-08-09

Schwartz, Richard A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 435215, 435226, 43525233, 4353201, 536 241, 935 44, C12P 2102, C12N 1567

Patent

active

053366026

ABSTRACT:
An expression enhancer has a DNA sequence which is capable of forming a t-RNA clover-leaf structure after transcription and hybridizes in that region of the DNA which, after transcription, forms the anticodon loop in the clover-leaf structure with an oligonucleotide with the sequence 5'-GACTTAGAAGGTCGTT-3' or its complementary sequence (5'-AACGACCTTCTAAGTC-3'). It can be used to increase the yield in the expression of a recombinant gene by transformation of suitable host cells with an expression vector containing the recombinant gene, whereby it is likewise introduced into the host cells in a form capable of expression and expressed.

REFERENCES:
Garcia et al., Cell v. 45, 1986, pp. 453-459.
Winnacker, From Genes to Clones, 1987, VCH, New York, pp. 276-279.
Pennica et al., Nature, v. 301, 1983, pp. 214-221.

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