Expression elements

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C435S007100, C435S320100, C435S325000, C435S354000, C435S358000, C435S363000, C435S367000, C536S024100

Reexamination Certificate

active

07632661

ABSTRACT:
The invention relates to genetic elements capable of improving the levels of expression of operably-linked transcription units. In particular, said genetic elements are derived from the 5′ untranslated regions of ribosomal protein genes and may comprise a CpG island. Also provided are vectors and host cells comprising said genetic elements and methods of use to obtain high levels of recombinant gene expression.

REFERENCES:
patent: 5583009 (1996-12-01), Palmiter et al.
patent: 5610053 (1997-03-01), Chung et al.
patent: 6063598 (2000-05-01), Enenkel et al.
patent: 6689606 (2004-02-01), Antoniou et al.
patent: 6881556 (2005-04-01), Antoniou et al.
patent: 6949361 (2005-09-01), Antoniou et al.
patent: 6964951 (2005-11-01), Antoniou et al.
patent: 7442787 (2008-10-01), Antoniou et al.
patent: 2003/0166890 (2003-09-01), Crombie et al.
patent: 2004/0148647 (2004-07-01), Enenkel et al.
patent: WO94/13273 (1994-06-01), None
patent: WO95/33841 (1995-12-01), None
patent: WO98/07876 (1998-02-01), None
patent: WO 00/05393 (2000-02-01), None
patent: WO 02/24930 (2002-03-01), None
patent: WO 02/081677 (2002-10-01), None
patent: WO 02/099089 (2002-12-01), None
patent: WO 03/006607 (2003-01-01), None
Lawrence S. Kirschner et al., “Structure Of The Human Ubiquitin Fusion Gene Uba80 (RPS27a) And One Of Its Pseudogenes,” Unit on Genetics and Endocrinology, Developmental Endocrinology Branch, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, Biochemical and Biophysical Research Communications 270, pp. 1106-1110 (2000), 0006-291X/00, http://www.idealibrary.com.
Daniel Finley et al., “The Tails Of Ubiquitin Precursors Are Ribosomal Proteins Whose Fusion To Ubiquitin Facilitates Ribosome Biogenesis,”Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, Nature vol. 338, Mar. 30, 1989, pp. 394-401.
Yuen-Ling Chan et al., “The Carboxyl Extensions Of Two Rat Ubiquitin Fusion Proteins Are Ribosomal Proteins S27a And L40,”Department of Biochemistry and Molecular Biology, The University of Chicago, Chicago, Illinois 60637, Biochemical And Biophysical Research Communications, vol. 215, No. 2, Oct. 13, 1995, pp. 682-690, 0006-291X/95.
Narayanan Hariharan et al., “Euipotent Mouse Ribosomal Protein Promoters Have A Similar Architecture That Includes Internal Sequence Elements,”Institute for Cancer Research, Fox Chase Cancer Center, Philadelphia, Pennsylvania 1911, Genes & Development 3: 1789-1800, 1989, ISSN 0890-9369/89.
Olivier Coux, et al., “Structure And Functions Of The 20S and 26S Proteasomes,” Annu. Rev. Biochem. 1996, 65:801-847, 1996.
Kent L. Redman et al., “The cDNA For The Ubiquitin-52-Amino-Acid Fusion Protein From Rat Encodes A Previously Unidentified 60 S Ribosomal Subunit Protein,” Biochem J. (1996) 315, pp. 315-321 (Printed in Great Britain).
Robert P. Perry, “The Architecture Of Mammalian Ribosomal Protein Promoters,”BioMed Central, BMC Evolutionary Biology, 2005, 5:15, http://www.biomedcentral.com/1471-2148/5/15.
Peter Gunning et al., A human B-actin expression vector system directs high-level accumulation of antisense transcripts, Proc. Natl. Acad. Sci, vol. 84, pp. 4831-4835, Jul. 1987.
M. Garidiner-Garden et al., CpG Islands in Vertebrate Genomes, J. Mol. Biol. 196, 261-282, 1987.
Bonifer, “Long-distance chromatin mechanisms controlling tissue-specific gene locus activation”, Gene, 238(2):277-289, Oct. 1999.
Anderson, “Human gene therapy”, Nature, 392(Supp):25-30, Apr. 1998.
Verma et al., “Gene therapy-promises, problems and prospects”, Nature, 389:239-242, Sep. 1997.
Corcoran et al., “High-level regulated expression of the human G6PD gene in transgenic mice”, Gene, 173(2):241-246, Sep. 1996.
Biamonti et al., “Two homologous genes, originated by duplication, encode the human hnRNP proteins A2 and A1”, Nucl. Acids Res., 22(11):1996-2002, Jun. 1994.
Genbank Accession No., U09120, National Library of Medicine, accessed by PTO, Jul. 5, 2000, Dec. 1994.
Genbank Accession No. D28877, National Library of Medicine, accessed by PTO, Jul. 5, 2000, Feb. 1999.
Genbank Accession No. AL031259, version AL031259.7 Gi:3676176, accessed by PTO, Jul. 13, 2000, Sep. 1998.
Chalut et al., “Genomic structure of the human TATA-box-binding protein (TBP)”, Gene, 161 (2):277-282, Aug. 1995.
Foulds et al., “Analysis of the human TATA binding protein promoter and identification of an Ets site orifice for activity”, Nucl. Acids Res., 25(12):2485-2494, Jun. 1997.
Li et al, “Locus control regions: coming of age at a decade plus”, Trends in Genetics, 15 (10):403-408, Oct. 1999.
Chung et. al.; Characterization of the chicken β-globin insulator, 1997, Proc..Natl. Acad. Sci. vol. 94: 575-580.
Crane-Robinson, C. et al., “Chromosomal mapping of core histone acetylation by immunoselection,” Methods, 1997, 12(1), 48-56 (summary only).
DiBartolomeis, S.M. et al., “A superfamily of Drosophila satellite related (SR) DNA repeats restricted to the X chromosome euchromatin,” Nucl. Acids Res., 1992, 20(5), 1113-1116.
Duhig, T. et al., “The Human Surfeit Locus,” Genomics, 1998, 52, 72-78.
Ellis, J. et al., “A dominant chromatin-opening activity in 5' hypersensitive site 3 of the human β-globin locus control region,”.EMBO J., 1996, 15(3), 562-568.
Ellis, J. et al., “Evaluation of β-globin gene therapy constructs in single copy transgenic mice,” Nucl. Acids Res., 1997, 26(6), 1296-1302.
Gaston, K. et al., “CpG methylation has differential effects on the binding of YY1 and Ets proteins to the bi-directional promoter of the Surf-1 and Surf-2 genes,” Nucl. Acids Res., 1995, 23(6), 901-909.
Gaston, K. et al., “YY1 is involved in the regulation of the bi-directional promotor of the Surf-1 and Surf-2 genes,” FEBS Letts., 1994, 347, 289-294.
Garson, K. et al., “Surf5: A Gene in the Tightly Clustered Mouse Surfeit Locus is Highly Conserved and Transcribed Divergently from the rpL7a (Surf 3) Gene,” Genomics, 1996, 30, 163-170.
Gayalas, A. et al., “Analysis of the chicken GPAT/AIRC bidirectional promoter for de novo purine nucleotide synthesis,” J. Biol. Chem., 1995, 270(5), 2403-2410.
Lavia, P. et al., “Coincident start sites for divergent transcripts at a randomly selected CpG-rich island of mouse,” EMBO J., 1987, 6, 2773-2779.
Ortiz. S.D. et al., “Adjacent DNA elements dominantly restrict the ubiquitous activity of a novel chromatin-opening region to specific tissues,” EMBO J., 1997, 16, 5037-5045.
Palmiter, “The elusive function of metallothioneins,” Proc. Natl. Acad. Sci. USA, 1998, 95, 8428-8430.
Talbot, D. et al., “The 5' flanking region of the rat LAP (C/EBPβ) gene can direct high-level position-independent, copy number-dependent expression in multiple tissues in transgenic mice,” Nucl. Acids Res., 1994, 22(5), 756-766.
Williams, T.J. et al., “The MES-1 Murine Enhancer Element is Closely Associated with the Heterogeneous 5' Ends of Two Divergent Transcription Units,” Mol. Cell. Biol., 1986, 6(12), 4558-4569.
Winston, J. H. et al., “An intron 1 regulatory region from the murine adenosine deaminase gene can activate heterologous promoters for ubiquitous expression in transgenic mice,” Som. Cell Mol. Genet., 1996, 22, 261-278.
Festenstein, R., et al., “Locus control region function and heterochromatic-induced position effect variegation,” Science, 1996, 271(23), 1123-1125.
Gavalas, a., et al., “Coexpression of two closely linked avian genes for purine nucleotide synthesis from a bidirectional promoter,” Mol. Cell Biol., 1983, 13(8), 4784-4792.
Huxley, C., et al., “The mouse surfeit locus contains a cluster of six genes associated with four Gp G-rich islands i

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