Expression control elements from the Lemnaceae family

Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives

Reexamination Certificate

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C536S023400, C536S023600, C800S298000, C800S295000, C800S278000, C800S288000, C435S468000, C435S419000

Reexamination Certificate

active

08034916

ABSTRACT:
Compositions and methods for regulating expression of nucleotide sequences of interest in a plant are provided. Compositions include novel nucleic acid molecules, and variants and fragments thereof, for expression control elements isolated from theLemnaceaeubiquitin, r-histone and chitinase genes. A method for expressing a nucleotide sequence of interest in a plant using the expression control elements disclosed herein is further provided. The method includes introducing into a plant or plant cell or nodule an expression construct comprising an expression control element of the present invention operably linked to a nucleotide sequence of interest. In particular, the compositions and methods find use in enhancing expression of nucleotide sequences of interest in duckweed. Also provided is a novelLemnaceaesignal peptide-encoding sequence and the signal peptide encoded thereby. Where an expression construct of the invention is designed to express a polypeptide of interest, this novel signal peptide-encoding sequence can be included within the expression construct of the invention to provide for extracellular secretion of the encoded polypeptide of interest.

REFERENCES:
patent: 5510474 (1996-04-01), Quail et al.
patent: 5614399 (1997-03-01), Quail et al.
patent: 6020190 (2000-02-01), Quail et al.
patent: 6054574 (2000-04-01), Quail et al.
patent: 6528701 (2003-03-01), Wang et al.
patent: 2003/0033630 (2003-02-01), Stomp et al.
patent: 2007/0006347 (2007-01-01), Plesch et al.
patent: 2007/0180583 (2007-08-01), Dickey et al.
Maiti et al. Promoter/leader deletion anaylsis and plant expression vectors with the figwort mosaic virus (FMV) full length transcript (FLt) promoter containing single or double enhancer domains. (1997) Transgen. Res.; vol. 6; pp. 143-156.
Doelling et al. The minimal ribosomal RNA gene promoter ofArabidopsis thalianaincludes a critical element at the transcription initiation site. (1995) Plant J.; vol. 8; pp. 683-692.
Donald et al. Mutation of either G box or I box sequences profoundly affects expression from theArabidopsisrbcS-1A promoter. (1990) EMBO J. vol. 9; pp. 1717-1726.
Benfey et al. The cauliflower mosaic virus 35S promoter: combinatorial regulation of transcription in plants. (1990) Science; vol. 250; pp. 959-966.
Kim et al. A 20 nucleotide upstream element is essential for the nopaline synthase (nos) promoter activity. (1994) Plant Mol. Biol. vol. 24; pp. 105-117.
Kehoe et al. Two 10-bp regions are critical for phytochrome regulation of a Lemna gibba Lhcb gene promoter. (1994) The Plant Cell; vol. 6; pp. 1123-1134.
Gillikin et al. A defective signal peptide tethers the floury-2 zein to the endoplasmic reticulum membrane. (1997) Plant Physiol.; vol. 114, pp. 345-352.
Stark et al. Two enhancers and one silencer located in the introns of a regA control somatic cell differentiation in Volvox carteri. (2001) Genes & Develop.; vol. 15; pp. 1449-1460.
Williams, S.A., “NPR Genes, Which are Negatively Regulated by Phytochrome Action in Lemma gibba L. G-3, Can Also Be Positively Regulated by Abscisic Acid,”Plant Physiol.,1994, pp. 949-954, vol. 105.
Benfey, P.N., and N-H Chua, “The Cauliflower Mosaic Virus 35 S Promoter: Combinatorial Regulation of Transcription in Plants,”Science,Nov. 16, 1990, pp. 959-966, vol. 250.
Chaubet-Gigot, Nicole, et al., “Tissue-Dependent Enhancement of Transgene Expression by Introns of Replacement Histone H3 Genes ofArabidopsis”, Plant Molecular Biology,2001, vol. 45, pp. 17-30.
Doeling, J.H., and C.S. Pikaard, “The Minimal Ribosomal RNA Gene Promoter ofArabidopsis thalianaIncludes a Critical Element at the Transcription Initiation Site,”Plant J.,1995, pp. 683-692, vol. 8, No. 5.
Donald, R.G.K., and A.R. Cashmore, “Mutation of Either G Box or I Box Sequences Profoundly Affects Expression from theArabidopsis rbcS-1APromoter,”The EMBO Journal,1990, pp. 1717-1726, vol. 9, No. 6.
Hondred, David, et al., “Use of Ubiquitin Fusions to Augment Protein Expression in Transgenic Plants”,Plant Physiology,Feb. 1999, vol. 119, pp. 713-723.
Kehoe, D.M., et al., “Two 10-bp Regions are Critical for Phytochrome Regulation of aLemna gibba LhcbGene Promoter,”Plant Cell,Aug. 1994, pp. 1123-1134, vol. 6, No. 8.
Kim, Y., et al., “A 20 Nucleotide Upstream Element is Essential for the Nopaline Synthase (nos) Promoter Activity,”Plant Molecular Biology,1994, pp. 105-117, vol. 24.
Maiti, I.B., et al., “Promoter/Leader Deletion Analysis and Plant Expression vectors with the Figwort Mosaic Virus (FMV) Full Length Transcript (FLt) Promoter Containing single or Double Enhancer Domains,”Transgenic Research,1997, pp. 143-156, vol. 6.
Ngsee, J.K., et al., “Cassette Mutagenic Analysis of the Yeast Invertase Signal Peptide: Effects on Protein Translocation,”Molecular and Cellular Biology,Aug. 1989, pp. 3400-3410, vol. 9, No. 8.
Plesse, B., et al., “Effects of the Polyubiquitin GeneUbi.U4Leader Intron and First Ubiquitin Monomer on Reporter Gene Expression inNicotiana tabacum”, Plant Molecular Biology,2001, vol. 45, pp. 655-667.
Rolfe, S.A., and E.M. Tobin, “Deletion Analysis of a Phytochrome-regulated Monocot rbcS Promoter in a Transient AssaySystem,”Proc. Natl. Acad. Sci.,Apr. 1991, pp. 2683-2686, vol. 88, No. 7.
Sivamani, Elumalai, et al., “Expression Enhancement of a Rice Polyubiquitin Gene Promoter”,Plant Molecular Biology,2006, vol. 60, pp. 225-239.
Sun, Chih-Wen, et al., “Independent Modulation ofArabidopsis thalianaPolyubiquitin mRNAs in Different Organs and in Response to Environmental Changes”,The Plant Journal,1997, vol. 11(5), pp. 1017-1027.
Wang, J., et al., “Rice Ubiquitin Promoters: Deletion Analysis and Potential Usefulness in Plant Transformation Systems”, 2003,Plant Cell Reports,pp. 1-16.
Wang, Jianlin, et al., Structure, Expression and Promoter Activity of Two Polyubiquitin Genes from Rice (Oryza sativa L.),Plant Science,2000, vol. 156, pp. 201-211.
Waterborg, Jakob, H., et al., “Common Features of Analogous Replacement Histone H3 Genes in Animals and Plants”,J. Mol. Evol.,1996, vol. 43, pp. 194-206.
Wei, Hairong, et al., “Comparative Expression Analysis of Two Sugarcane Polyubiquitin Promoters and Flanking Sequences in Transgenic Plants”,J. Plant Physiol.,2003, vol. 160, pp. 1241-1251.
EMBL Database Accession No. AL161589, submitted on Mar. 10, 2000 (XP002459417).

LandOfFree

Say what you really think

Search LandOfFree.com for the USA inventors and patents. Rate them and share your experience with other people.

Rating

Expression control elements from the Lemnaceae family does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Expression control elements from the Lemnaceae family, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression control elements from the Lemnaceae family will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFUS-PAI-O-4291945

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.