Expression cassettes useful in construction of integrative and r

Details Expression cassettes useful in construction of integrative and r Expression cassettes useful in construction of integrative and r

1994-06-23

1997-04-22

Elliott, George C.

Chemistry: molecular biology and microbiology

Vector, per se

435 691, 435 712, 435 713, 4351721, 4351723, 43525235, 536 232, C12N 1563, C12N 1500, C12N 120, C07H 2104

Patent

active

056228660

ABSTRACT:
The present invention is directed to expression cassettes useful in gene expression studies in both homologous and heterologous Streptomyces strains. More specifically the present invention is directed to the construction of two Streptomyces lividans-recombinant strains capable of producing 31-O-desmethylFK-506 O:methyltransferase, which methvlates 31-O-desmethylFK-506 to FK-506. In addition, the present invention is directed to a process for the specific methylation of 31-desmethyl-FK506 to FK506.

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Pouwels, et al., in Cloning Vectors, Elsevier, (1985) pp. IIIA-i-6--IIIA-ii-1.
Omer et al., T. Bacteriol. 170(5):2174-2184 (1988).

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