Expression cassettes for root-preferential expression in plants

Multicellular living organisms and unmodified parts thereof and – Method of introducing a polynucleotide molecule into or... – The polynucleotide contains a tissue – organ – or cell...

Reexamination Certificate

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C800S278000, C536S024100, C435S320100

Reexamination Certificate

active

07999150

ABSTRACT:
The present invention relates to expression cassettes comprising transcription regulating sequences with root-preferential or root-specific expression profiles in plants obtainable fromArabidopsis thalianagenes At1g66280, At1g74500, At1g49570, At1g70710, At5g66690, At3g29630, At5g48070, or At4g17800. The expression cassettes according to the present invention may be employed for expression of a protein, or expression of an antisense RNA, sense or double-stranded RNA. Preferably, expression of the nucleic acid sequence confers to the plant an agronomically valuable trait. Vectors comprising such expression cassette, and transgenic host cell or non-human organism comprising the expression cassette or the vector is also enclosed. Also enclosed is a method for identifying and/or isolating a sequence with root-specific or root-preferential transcription regulating activity.

REFERENCES:
patent: 5459252 (1995-10-01), Conkling et al.
patent: 5466785 (1995-11-01), de Framond
patent: 5633363 (1997-05-01), Colbert et al.
patent: 5750386 (1998-05-01), Conkling et al.
patent: 5837848 (1998-11-01), Ely et al.
patent: 6018099 (2000-01-01), deFramond
patent: WO-02/38588 (2002-05-01), None
patent: WO-03/074688 (2003-09-01), None
Genbank Accession No. AB017064 (1999).
“Arabidopsis thaliana hydrolase, hydrolyzing o-glycosyl compounds AT1G66280 mRNA, complete cds.”, May 13, 2003, GenBank Accession No. NM—105299.
“Arabidopsis thaliana DNA binding / transcription factor AT1G74500 mRNA, complete cds.”, Nov. 4, 2005, GenBank Accession No. NM—106110.
“Hydrolase, hydrolyzing o-glycosyl compounds [Arabidopsis thaliana].”, Nov. 4, 2005, GenBank Accession NP—176802.
“DNA binding / transcription factor [Arabidopsis thaliana].”, Nov. 4, 2005, GenBank Accession No, NP—177590.
“Arabidopsis thalianaperoxidase AT1G49570 mRNA, complete cds.”, May 13, 2003, GenBank Accession No. NM—103845.
“Peroxidase [Arabidopsis thaliana].” May 13, 2003, GenBank Accession No. NP—175380.
“Arabidopsis thalianaCEL1; hydrolase, hydrolyzing o-glycosyl compounds AT1G70710 (CEL1) mRNA, complete cds.”, May 14, 2003, GenBank Accession No. NM—105739.
“CEL1; hydrolase, hydrolyzing o-glycosyl compounds [Arabidopsis thaliana].”, Nov. 4, 2005, GenBank Accession No. NP—177228.
“Arabidopsis thalianaUDP-glycosyltransferase/ transferase, transferring glycosyl groups AT5G66690 mRNA, complete cds.”, May 13, 2003, GenBank Accession No. NM—126067.
“UDP-glycosyltransferase/transferase, transferring glycosyl groups [Arabidopsis thaliana],”, Nov. 4, 2005, GenBank Accession No. NP—201470.
“Arabidopsis thalianatransferase, transferring glycosyl groups AT3G29630 mRNA, complete cds.”, May 13, 2003, GenBank Accession No. NM—113884.
“Transferase, transferring glycosyl groups [Arabidopsis thaliana],”, Nov. 4, 2005, GenBank Accession No. NP—189604.
“Arabidopsis thalianaATXTH2O; hydrolase, acting on glycosyl bonds / hydrolase, hydrolyzing o-glycosyl compounds AT5G48070 (ATXTH2O) mRNA, complete cds.”, May 13, 2003, GenBank Accession No. NM—124181.
“ATXTH20; hydrolase, acting on glycosyl bonds / hydrolase, hydrolyzing o-glycosyl compounds [Arabidopsis thaliana].”, Nov. 4, 2005, GenBank Accession No, NP—199618.
“Arabidopsis thalianaunknown protein AT4G17800 mRNA, complete cds.”, Feb. 17, 2004, GenBank Accession No. NM—117890.
“Unknown protein [Arabidopsis thaliana].”, Nov. 4, 2005, GenBank Accession No. NP—193515.
Hwang I. et al., “Arabidopsis thalianaroot-specific kinase homolog is induced by dehydration, ABA, and NaCl.” The Plant Journal, 1995, vol. 8(1), pp. 37-43.
Malboobi, M.A. et al., “A phosphate-starvation indicible β-glucosidase gene (psr3.2) isolated fromArabidopsis thalianais a memeber of a distinct subfamily of the BGA family”, Plant Molecular Biodiogy , 1997, vol. 34, pp. 57-68.
Nitz, I. et al., “Pyk 10, a seedling and root specific gene and promoter fromArabidopsis thaliana”, Plant Science, 2001, pp. 337-346.
Müssig, C. et al., “Changes in gene expression in response to alteredSHLtranscript levels”, Plant Molecular Biology, 2003, vol. 53, pp. 805-820.
Ahn, Y.O. et al., “Furcatin hydrolase fromViburnum furcatumblume is a novel disaccharide-specific acuminosidase in glycosyl hydrolase family 1”, The Journal of Biological Chemistry, 2004, vol. 279, No. 22, pp. 23405-23414.
“Beta-glucosidase, putative; 11384-8406”, Jun. 1, 2001, GenBank Accession No. Q9C8Y9.
“Arabidopsis thalianachromosome 1 BAC T27F4 genomic sequence, complete sequence”, Jan. 11, 2000, GenBank Accession No. AC020665.
Oommen, A, et al., “The elicitor-inducible alfalfa isoflavone reductase promoter confers different patterns of developmental expression in homologous and heterologous transgenic plants”, Plant Cell, 1994, vol. 6, No. 12, pp. 1789-1803.
Fourgoux-Nicol, A., et al. “Isolation of rapeseed genes expressed early and specifically during development of the male gametophyte”, Plant Mol Biol., 1999, vol. 40, No. 5, pp. 857-872.
Kim, Y., et al., “A 20 nucleotide upstream element is essential for the nopaline synthase (nos) promoter activity”, Plant Mol Biol., 1994, vol. 24, No. 1, pp. 105-117.
Suzuki, H., et al., “Deletion analysis and localization of SbPRP1, a soybean cell wall protein gene, in roots of transgenic tobacco and cowpea”, Plant Mol Biol., 1993, vol. 21, No. 1, pp. 109-119.
Padgette, S. R., et al., “Development, identification, and characterization of a glyphosphate-tolerant soybean line”, Crop Sci., 1995, vol. 35, pp. 1451-1461.
An, G., et al., “Transformation of Tobacco, Tomato, Potato, andArabidopsis thalianaUsing a Binary Ti Vector System”, Plant Physiol., 1986, vol. 81, No, 1, pp. 301-305.
“Arabidopsis thalianachromosome 1 BAC F1M20 genomic sequence, complete sequence”, Jan. 19, 2001, GenBank Accession No. AC011765.
“Arabidopsis thalianaclone 519 mRNA, complete sequence”, Jan. 27, 2006, GenBank Accession No, AY088286.

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