Expression cassette operable in a recombinant host

Details Expression cassette operable in a recombinant host Expression cassette operable in a recombinant host

1995-04-06

1999-02-09

Elliott, George C.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

4351723, 435190, 435233, 4352523, 43525231, 43525233, 4352542, 43525421, 4353201, 435325, 536 232, 536 235, C12N 1512, C12N 1555, C12N 1561, C12P 3300

Patent

active

058692835

ABSTRACT:
An expression cassette, operable in a recombinant host, comprising a heterologous DNA coding sequence encoding a protein, which is functional, alone or in cooperation with one or more additional proteins, of catalyzing an oxidation step in the biological pathway for conversion of cholesterol into hydrocortisone, which step is selected from the group consisting of:

REFERENCES:
Zuber et al. Expression of bovine 17alpha-hydroxylase cytochrome P-450 cDNA in nonseroidogenic (COS 1) cells. Science 234: 1258-1261, Dec. 1986.
The et al. Full length cDNA structure and deduced amino acid sequence of human 3 beta-hydroxy-5-ene steroid dehydrogenase. Molecular Endocrinology 3(8): 1310-1312, Aug. 1989.

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