Expression and secretion vector in yeasts, useful for preparing

Details Expression and secretion vector in yeasts, useful for preparing Expression and secretion vector in yeasts, useful for preparing

1993-06-01

1995-07-11

Patterson, Jr., Charles L.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Bacteria or actinomycetales; media therefor

43525521, 4353201, 435 6952, 935 28, 935 48, C12N 1570, C12N 1581, C12N 1925

Patent

active

054320828

ABSTRACT:
The invention provides an expression and secretion vector in yeasts which is useful for preparing heterologous proteins, comprising a synthetic oligonucleotide which directs the secretion of the heterologous protein wherein the synthetic oligonucleotide is positioned between the inducible hybrid promoter GAL-CYC and a multiple-site polylinker followed by the signals of transcription termination recognized by the RNA polymerase of the yeasts. The invention includes a hybrid plasmid obtained by cloning in one of the restriction sites of the polylinker of the vector, the DNA sequence which codes for a heterologous protein. A process is also described for the preparation of heterologous proteins which comprises cultivating in a suitable culture medium a yeast transformed with the hybrid plasmid and recovering from the culture medium the resulting heterologous proteins. The recovered proteins include hormones, lymphokines, viral antigens or immunogens useful in the therapeutical and diagnostic field.

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Stark, M. J. R., et al., (1984) Nucl. Acids Res 12(15), 6011-6024.
Stark, M. J. R., et al., (1986) Embo J. 5(8), 1995-2002.
Innis, M. A., in Yeast Genetic Engineering, eds., Barr, et al., (Butterworths, Boston) Chap. 12 pp. 233-246.
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