Expression and secretion vector for yeast containing a glucoamyl

Details Expression and secretion vector for yeast containing a glucoamyl Expression and secretion vector for yeast containing a glucoamyl

1985-12-18

1991-06-18

Teskin, Robin L.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

435691, 435698, 435202, 435203, 435204, 435205, 435255, 435256, 4353201, 536 27, 935 47, 935 48, C12P 2100, C12N 1500, C12N 503

Patent

active

050249410

ABSTRACT:
A vector including a DNA sequence encoding a secretory signal sequence substantially identical to the secretory signal-encoding sequence of a glucoamylase gene from Saccharomyces diastaticus or S. cerevisiae; and upstream from the signal-encoding sequence, a DNA sequence capable of promoting transcription in yeast (e.g., a high yield promoter, such as the promoter of the triose phosphate isomerase gene), transcription of the signal-encoding sequence being under the control of the transcription-promoting sequence, a site for the insertion into the vector of a heterologous DNA sequence, in reading frame with the signal-encoding sequence. The vector is useful as an expression vector in yeast.

REFERENCES:
patent: 4546082 (1985-10-01), Kurjan
patent: 4727028 (1988-02-01), Santerre et al.
Smith, R. A. et al.; Heterologous Protein Secretion from Yeast; Science 229, pp. 1219-1223 (1985).
Innis, M. A. et al.; Expression, Glycosylation and Secretion of an Aspergillus Glucoamylase by Saccharomyces Cerevisiae, Science, 228, pp. 21-26 (1985).
Yamashita, I., and S. Fukui; Secretion of S. Diastatrus Glucoamylase from S. Ponbe; Agric. Biol. Chem 48, pp. 1931-1932 (1984).
Meaden, P. et al.; A Dex Gene Conferring Production of Extracellular Amyloglucosidase on Yeast; Gene 34, pp. 325-334 (1985).
Yamashita, I. et al.; Cloning and Expression of the S. fiyuligera glucoamylase gene in S. cerevisiae, Appl. Microbiol Biotechnol 23; pp. 130-133 (1985).
PCT; Nunberg, J. H. et al.; Glucoamylase cDNA; Aug. 2, 1984.
Yamashita, I. and S. Fukui; Molecular Cloning of a Glucoamylase-Producing Gene in the Yeast Succharomyces; Agric. Biol. Chem. 47, pp. 2689-2692.
Yamashita, I. et al.; Nucleotide Sequence of the Extracellular Glycoamylase Gene STA1 in the yeast S. Diastaticus; J. Bacteriol 161, pp. 567-573 (1985).
Yamashita, I. et al.; Polymorphic Extracellular Glycoamylase Genes and Their Evolutionary Origin in the Yeast S. Diastaticus; J. Bacteriol. 161, pp. 574-582 (1985).
Brown et al., (1984), M.G.G. 197:351.
Smith et al. (1985) Science, 229: 1219.
Taussig et al. (1983), Nucl. Acid Res. 11:1943.

Affiliated with

Also associated with

Rating

Expression and secretion vector for yeast containing a glucoamyl does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Expression and secretion vector for yeast containing a glucoamyl, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression and secretion vector for yeast containing a glucoamyl will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-145374