Expression and secretion vector for human interferon alpha...

Details Expression and secretion vector for human interferon alpha... Expression and secretion vector for human interferon alpha...

2006-05-30

2006-05-30

Andres, Janet L. (Department: 1647)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S320100, C435S069510

Reexamination Certificate

active

07052867

ABSTRACT:
Disclosed in this invention are an expression vector for the secretive production of human interferon alpha (hIFNα) comprising a polynucleotide encoding a modifiedE. colithermostable enterotoxin II signal sequence and a polynucleotide encoding hIFNα ligated to the 3′-end thereof; a microorganism transformed with the expression vector; and a process for secretively producing human interferon by culturing the microorganism, the process being capable of secreting a soluble form of active hIFNα, which does not contain an additional methionine residue at its N-terminal, into the periplasm of anE. colicell.

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