Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1986-03-18
1990-06-26
Teskin, Robin L.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 698, 4351723, 435255, 435256, 435259, 4351721, 435320, 435 91, 435116, 435 699, 935 28, 935 37, 935 56, 935 69, 536 27, C12P 2100, C12P 1934, C12N 500
Patent
active
049371894
ABSTRACT:
Sequencing of the XPR2 and LEU2 genes of Yarrowia lipolytica, recombinant Yarrowia lipolytica cloning vehicles comprising heterologous DNA coding for the expression of mammalian protein and other polypeptides, including plasmids suited for transformation of Y. lipolytica hosts and incorporating a regulon homologous to the host in its untransformed state, and secretion signals for the heterologous gene; integrative expression vectors using the XPR 2 gene promoter, alkaline protease pre-proregion and XPR2 terminator region and those having the LEU2 promoter and alkaline protease secretory signal sequences capable, in a transformed Y. lipolytica cell culture, of expressing and secreting a heterologous protein outside the cell; Y. lipolytica transformants comprising said vectors and plasmids; methods for preparing vectors to direct secretion of specified heterologous proteins coded for by genes, cDNA or synthetic DNA in Y. lipolytica in their mature, functional state.
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Davidow Lance S.
DeZeeuw John R.
Franke Arthur E.
Benson Gregg C.
Lumb J. Trevor
Pfizer Inc.
Richardson Peter C.
Teskin Robin L.
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