Chemistry: molecular biology and microbiology – Micro-organism – per se ; compositions thereof; proces of... – Bacteria or actinomycetales; media therefor
Patent
1996-03-12
1999-11-02
Wax, Robert A.
Chemistry: molecular biology and microbiology
Micro-organism, per se ; compositions thereof; proces of...
Bacteria or actinomycetales; media therefor
435 691, 43525233, 4353201, 536 231, 536 234, 536 237, C12N 122, C07H 2104
Patent
active
059768649
ABSTRACT:
DNA constructs are provided which code for at least the extreme C-terminal amino acids of the rsaA protein of Caulobacter crescentus fused with heterologous polypeptides. Baterial cells containing, or which express the DNA constructs and secrete the resulting protein are also provided. Chimeric proteins including the C-terminal amino acids of the rsaA protein are provided, including chimeric proteins comprising antigenic epitopes of the Infectious Hematopoietic Necrosis Virus.
REFERENCES:
Nomellini J. et al., "Insertion of Heterologous Peptides within the Surface-Layer Protein of Caulobacter Crescentus", Abstracts of the General Meeting of the American Society for Microbiology, vol. 95, 1995, p. 525, XP002035105.
Database WPI, "Production of Heterologous Polypeptides in Bacteria, Particularly Caulobacter-by Expression of a Fusion product of the Polypeptide Sequence and a Bacterial S-layer Protein Gene" & CA2090549 (University of British Columbia), Dec. 10, 1993, Section Ch, Week 9409, Derwent Publications Ltd., London, GB; Class C12, p. 027, AN 94-066249 XP002035014.
Koener J. et al., "Nucleotide Sequence of Gene of Infectious Hematopoetic Necrosis Virsus, a Fish Rhabdovirus" cited in the application, Journal of Virology, vol. 61, 1987, pp. 1342-1349, XP002034944.
Bingle W. et al., "Linker Mutagenesis of the Caulobacter Crescentus S-layer Protein: Toward a Definition of an N-terminal Anchoring Region and a C-terminal Secretion Signal and the Potential for Heterologous Protein Secretion", Journal of Bacteriology, vol. 179, No. 3, Feb. 1997, pp. 601-611, XP002034945.
Bingle W. et al., "The Extreme N-Terminus of the Caulobacter Crescentus Surface-Layer Protein Directs Export of Passenger Proteins From the Cytoplasm But is not Required for Secretion of teh Native Protein", Canadian Journal of Microbiology, vol. 42, No. 7, Jul. 1996, pp. 672-684, XP002034946.
Smit, John; and, Nina Agabian; "Cloning of the Major Protein of the Caulobacter crescentus Periodic Surface Layer: Detection and Characterization of the Cloned Peptide by Protein Expression Assays" (1984) J. Bacteriol. 160, 1137-1145.
John Smit, "Protein Surface Layers of Bacteria", in: Bacterial Outer Membranes as Model Systems (1986), John Wiley & Sons, Inc. (M. Inouye, ed.); at pp. 344-376.
Fisher, James A.; John Smit; and, Nina Agabian; Transcriptional Analysis of the Major Surface Array Gene of Caulobacter crescentus: (1988) J. Bacteriol 170, 4706-4713.
Charbit, Alain; et al; "Versatility of a Vector for Expressing Foreign Polypeptides at the Surface of Gram-Negative Bacteria" (1988) Gene 70, 181-189.
Koener, J.F., et al, "Nucleotide Sequence of a cDNA Clone Carrying the Glycoprotein Gene of Infectious Hematopoietic Necrosis Virus, a Fish Rhabdovirus" (1987) 61 J. Virology, pp. 1342-1349, at p. 1345.
Bingle, Wade H.; and, John Smit; "High-Level Expression Vectors for Caulobacter crescentus Incorporating the Transcription/Translation Initiation Regions of the Paracrystalline Surface-Layer-Protein Gene" (1990) Plasmid 24, 143-148.
Gilchrist, A. and Smith, J., "Transformation of Freshwater and Marine Caulobacters by Electroporation" (1991), 173 J. Bacteriology, pp. 921-925.
Pugsley, A.P. (1991). Superfamilies of bacterial transport systems with nucleotide binding components. In Prokaryotic Structure and Function: A New Perspective. Edited by S. Mohan, C. Dow, and J.A. Coles. Cambridge University Press, New York. pp. 223-248.
Hayes, L.J.; et al; "Chlamydia trachomatis Major Outer Membrane Protein Epitopes Expressed as Fusions with LamB in an Attenuated aroA Strain of Salmonella typhimurium; Their Application as Potential Immunogens" (1991) J. General Microbiology 137, 1557-1564.
Smit, John; and, James Atwater; "Use of Caulobacters to Separate Toxic Heavy Metals from Wastewater Streams" (Apr., 1991) U.S. Department of Energy Publication.
Smit, J. and Atwater, J., Use of Caulobacters to Separate Toxic Heavy Metals From Wastewater Streams, in: Proceedings of Waste Stream Management & Utilization Innovative Concepts--An Experimental Technique Exchange (vol. 1) Mining & Metals Remediation, Wash. D.C. Apr. 25-26, 1991, U.S. Department of Energy, Pacific Northwest Lab, Richland, Washington; at pp. 6.1-6.11.
Federal Grant is First for Manmade Cleanup Bug, in: Environment Today, vol. 2, Nov./Dec. 1991.
P. Edwards and J. Smit, "A transducing Bacteriophage for Caulobacter crescentus Uses the Paracrystalline Surface Layer Protein as a Receptor" (1991) 173 J. Bacteriology, pp. 5568-5572.
W.H. Bingle and J. Smit, "A Method of Tagging Specific-Purpose Linkers with an Antibiotic-Resistance Gene for Linker Mutagenesis Using a Selectable Marker", (1991) 151 BioTechniques, pp. 150-152.
Walker, S.G. et al, :Isolation and Comparison of the Paracrystalline Surface Layer Proteins of Freshwater Caulobacters, (1992) 174 J. Bacteriology, pp. 1783-1792.
Bingle, W.H., et al, "Caulobacters as Potential Biological Agents for Treatment of Pulp and Paper Effluent: Sequestration of Heavy Metals and Depolymerization of Cellulose", in: BioforBiogual '92, Vancouver, Canada, Jun. 9-11, 1992.
Gilchrist, Angus; J.A. Fisher; and, J. Smit; "Nucleotide Sequence Analysis of the Gene Encoding the Caulobacter crescentus Paracrystalline Surface Layer Protein" (Mar., 1992) Can. j. Microbiol 38, 193-202.
Bingle, W.H. et al, "Definition of Form and Function for the S-Layer of Caulobacter crescentus" in: Advances in Bacterial Paracrystalline Surface Layers (1993) Plenum Press (T.J. Beveridge and S.F. Koval ed.), pp. 181-197 (see enclosed author's copy insert at pp. 9-11).
Bingle, W.H., et al, Linker Mutagenesis of the Caulobacter crescentus S-Layer Protein:, in: Advances in Bacterial Paracrystalline Surface Layers (1993) Plenum Press (T.J. Beveridge and S.F. Koval ed.), pp. 293-296.
T.J. Beveridge, et al, "Summary Statements", in: Advances in Bacterial Paracrystalline Surface Layers (1993) Plenum Press (T.J. Beveridge and S.F. Koval, ed.), pp. 323-327; at p. 325.
Pugsley, A.P. 1993. The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57: 50-108.
Leong, J.A. 1993. Molecular and biotechnological approaches to fish vaccines. Curr. Opin. Biotech. 4:286-93.
Bingle, W.H., Kurtz, H.D., Jr., et Smit, J. 1993. An "all-purpose" cellulase reporter for gene fusion studies and application to the paracrystalline surface (S)-layer protein of Caulobacter crescentus. Can. J. Microbiol. 39 : 70-80.
Bingle, W.H., et Smit, J. 1994. Alkaline phosphatase and a cellulase reporter protein are not exported from the cytoplasm when fused to large N-terminal portions of the Caulobacter crescentus surface (S)-layer protein. Can. J. Microbiol 40: 777-782.
Walker, Stephen G. et al.; Characterization of Mutants of Caulobacter crescentus Defective in Surface Attachment of the Paracrystalline Surface Layer (1994) J. Bacteriol. vol. 176, No. 20, at 6312-6323.
J. Smit et al; Annual meeting of American Society for Microbiology--Poster Presentation, Washington D.C., May 24, 1995.
Bingle Wade H.
Nomellini John F.
Smit John
Nashed Nashaat T.
The University of British Columbia
Wax Robert A.
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