Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Patent
1993-10-15
1995-12-12
Walsh, Stephen G.
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
435 691, 435199, 530413, C12N 916, C12N 920, C12N 1555, C07K 122
Patent
active
054749212
ABSTRACT:
The present invention is directed to an assay which is used to determine the inhibitory activity of a test compound against a particular phosphoinositide-specific phospholipase C enzyme, that enzyme being phospholipase C.gamma.. The present invention is also directed to the preparation of phospholipase C.gamma. by recombinant expression in a bacterial cell and isolation of the expressed enzyme.
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Koblan Kenneth S.
Pompliano David L.
Daniel Mark R.
Merck & Co. , Inc.
Muthard David A.
Walsh Stephen G.
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