Expression and purification of human interleukin-3 and muteins t

Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Lymphokines – e.g. – interferons – interlukins – etc.

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530412, 530413, 530415, 530417, 530418, 530419, 530420, 435 695, 435 6952, 930141, C07K 328

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053046374

ABSTRACT:
Methods are provided for improved production of hIL-3 either in glycosylated form from mammalian and yeast cells or in unglycosylated form from prokaryotes. Recombinantly produced human IL-3 is purified in a series of steps, initially employing hydrophobic interaction, followed by ion exchange chromatography and gel filtration.

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