Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1990-09-10
1992-12-29
Schwartz, Richard A.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 5, 435 712, 4351721, 4351723, 4352523, 43525233, 4353201, 435974, 536 27, 935 9, 935 12, 935 27, 935 72, 935 73, 930220, C12N 1549, C12N 1500, C12P 2100, C12P 2102
Patent
active
051750970
ABSTRACT:
Immunologically reactive gag proteins of LAV/HTLV-III are expressed in bacterial cells. The gag proteins are encoded by a recombinant plasmid containing procaryotic transcriptional and translational signals for expression, followed downstream by a DNA sequence comprising pGAG-1. Preferred signals for expression are selected from an inducible and/or suppressible operon, such as the trp operon. The gag proteins are isolated from the bacterial host and are utilized in diagnostic assays which detect the presence of LAV/HTLV-III antigens or antibodies immunologically reactive with LAV/HTLV-III. Further, the proteins produced by the method disclosed may be used as a vaccine against infection by the causative virus for acquired immune deficiency syndrome.
REFERENCES:
Tacon et al. (1980) Molec. Gen. Genet. 177:427-438.
Crowl et al. (1985) Cell 41:979-986.
Ratner et al. (1985) Nature 313:277-284.
F. Barre-Sinoussi et al., "Isolation of a T-Lymphotropic Retrovirus from a Patient at Risk for Acquired Immune Deficiency Syndrome (AIDS)", Science 220:868-871, 1983.
M. Popovic et al., "Detection, Isolation, and Continuous Production of Cytopathic Retroviruses (HTLV-III) from Patients with AIDS and Pre-AIDS", Science 224:497-500, 1984.
J. A. Levy et al., "Isolation of Lymphocytopathic Retroviruses from San Francisco Patients with AIDS", Science 225:840-842, 1984.
E. Vilmer et al., "Isolation of New Lymphotropic Retroviruses from Two Siblings with Haemophilia B, One with AIDS", Lancet i:753-757, 1984.
L. Wain-Hobson et al., "Nucleotide Sequence of the AIDS Virus, LAV", Cell 40:9-17, 1985.
M. A. Muesing et al., "Nucleic Acid Structure and Expression of the Human AIDS/Lymphadenopathy Retrovirus", Nature 313:450-458, 1985.
R. Sanchez-Pescador et al., "Nucleotide Sequence and Expression of an AIDS-Associated Retrovirus (ARV-2)", Science 227:484-492, 1985.
J. Schupbach et al., "Serological Analysis of a Subgroup of Human T-Lymphotropic Retroviruses (HTLV-III) Associated with AIDS", Science 224:503, 1984.
J. Schupbach et al., "Antibodies to HTLV-III in Swiss Patients with AIDS and Pre-AIDS and in Groups at Risk for AIDS", New Engl. J. Med. 312:265-270, 1985.
V. S. Kalyanaraman et al., "Antibodies to the Core Protein of Lymphadenopathy-Associated Virus (LAV) in Patients with AIDS", Science 225:321-323, 1984.
Cosand Wesley L.
McArdle Susan
Ward Pamela J.
Watanabe Susan M.
Genetic Systems Corporation
Railey II Johnny F.
Schwartz Richard A.
LandOfFree
Expression and diagnostic use of GAG-1 encoded peptides which ar does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Expression and diagnostic use of GAG-1 encoded peptides which ar, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Expression and diagnostic use of GAG-1 encoded peptides which ar will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1886651