Export systems for recombinant proteins

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...

Reexamination Certificate

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C425S069000, C425S071000, C425S320000, C536S023100, C536S023700, C536S024100

Reexamination Certificate

active

07129060

ABSTRACT:
The present invention relates to vectors, host-vector combinations and processes for preparing stable fusion proteins consisting of a carrier protein and a passenger protein, where expression of the fusion proteins leads to exposure of the passenger domains on the surface of bacterial cells, especiallyEscherichia colicells. If required, the passenger domains can be released into the medium by proteases, for example by selected host factors such as, for example, OmpT.

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Benz et al (Infection and Immunity, May 1989, p. 1506-1511).
Benz et al (Molecular Microbiology, 1992, 6(11), 1539-1546).
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Schaffer et al (Biochemie 83, 2001, pp. 591-599).
Benz et al (Molecular Microbiology, 220, 45(2), p. 267-276).

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